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Plasmid#154271PurposeBacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationbacterial codon optimizedPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJY3919
Plasmid#113332PurposeHermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoterDepositorInsertsbeta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 RNAiR2
Plasmid#51585Purposeexpresses RNAi-resistant (RNAiR) myc-tagged mouse Rem2 (long version)DepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationsilent mutations at shRNA 1 and 2 recognition sit…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJY3948
Plasmid#113338Purpose6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expressionDepositorInsert2-pyrone synthase, codon optimized for Y. lipolytica
UseTags6XHisExpressionYeastMutationPlease see depositor commentsPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDTN3997
Plasmid#113337PurposeMulti-copy 2-pyrone synthase plasmid for triacetic acid lactone production in Yarrowia lipolyticaDepositorInsertmulti-copy 2-pyrone synthase
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 S129N
Plasmid#51587Purposeexpresses RNAiR myc-tagged Rem2 non-GTP bindingDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationS129N; silent mutations at shRNA 1 and 2 recognit…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 L317G
Plasmid#51586Purposeexpresses RNAiR myc-tagged Rem2 non-CaM interactingDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationL317G; silent mutations at shRNA 1 and 2 recognit…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
CSII-mApple
Plasmid#207350PurposeMammalian expression of mAppleDepositorInsertmApple
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTD3685
Plasmid#113391Purposealpha factor inducible Ty3DepositorInsertTy3
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT3928
Plasmid#113331Purposecontrol for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoterDepositorInsertsbeta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNB2629
Plasmid#113394PurposeMET25p-MS2-RFP-CYC1 TTDepositorInsertMS2 bacteriophage coat protein
UseTagsred fluorescent protein (RFP), codon-optimized fo…ExpressionYeastMutationPromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKN3097
Plasmid#113392PurposeGalactose-inducible Ty3 with extra PPT- used for random bar code libraryDepositorInsertTy3
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 S69A
Plasmid#51590Purposeexpresses myc-tagged Rem2 S-A mutantDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationSerine 69 to Alanine; also RNAi resistantPromoterCMVAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 RNAiR2 short
Plasmid#51596Purposeexpresses RNAiR myc-tagged rat Rem2 (short version, missing 69 AA at N terminus)DepositorInsertRem2 (Rem2 Rat)
UseTagsmycExpressionMammalianMutationsilent mutations at shRNA 1 and 2 recognition sit…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 S308A
Plasmid#51592Purposeexpresses myc-tagged Rem2 S-A mutantDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationSerine 308 to Alanine; also RNAi resistantPromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 S241A
Plasmid#51591Purposeexpresses myc-tagged Rem2 S-A mutantDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationSerine 241 to Alanine; also RNAi resistantPromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 R236A/L263A
Plasmid#51589Purposeexpresses RNAiR myc-tagged Rem2 non-Ca channel interactingDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationR236A and L263A; silent mutations at shRNA 1 and …PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Rem2 S69A/S334A
Plasmid#51588Purposeexpresses RNAiR myc-tagged Rem2 non-14-3-3 interactingDepositorInsertRem2 (Rem2 Mouse)
UseTagsmycExpressionMammalianMutationS69A and S334A; silent mutations at shRNA 1 and 2…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKOR1-mcs
Plasmid#181755PurposeAllele replacement for Staphylococcus aureusDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4D
Plasmid#51599Purposeexpresses mouse Sema4D with N-terminal myc tagDepositorInsertSema4D (Sema4d Mouse)
UseTagsmyc tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only