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Showing: 1481 - 1500 of 6257 results

  1. pDONR223-ZNF69

    Plasmid
    #88143
    Purpose
    Donor Vector containing ZNF69 transcription factor, part of the Human TFome Collection
    Depositor
    Marc Vidal
    Article
    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.
    Insert
    ZNF69 (ZNF69 Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    Last nucleotide of stop codon removed to allow fo…
    Promoter
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    88143-plasmid-map-sequence-id-199028

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  5. AAVS1-SAGFP

    Plasmid
    #127100
    Purpose
    SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    SA-GFP reporter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127100-plasmid-map-sequence-id-247555

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  6. pINO4-Pfv-Sapphire-HO

    Plasmid
    #203659
    Purpose
    Yeast vector encoding donor DNA for integration of 40nm GEMs into the HO locus.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    CRISPR
    Tags
    Sapphire
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203659-plasmid-map-sequence-id-402489

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  7. pFHMSP-LIC-N

    Plasmid
    #26095
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Sept. 15, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

    Plasmid
    #121174
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121174-plasmid-map-sequence-id-234993

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  9. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

    Plasmid
    #121175
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121175-plasmid-map-sequence-id-235290

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  10. pLP-TRE2-hSox2

    Plasmid
    #49392
    Purpose
    inducible (dox) expression of hSox2
    Depositor
    Randall McKinnon
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Donor vector
    Tags
    6xHN
    Expression
    Mammalian
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    Tet-responsive
    Available since
    March 31, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pINO4-NLS-Pfv-Sapphire-HO

    Plasmid
    #203660
    Purpose
    Yeast vector encoding donor DNA for integration of nuclear 40nm GEMs into the HO locus.
    Depositor
    Liam Holt
    Article
    Shu T et et al bioRxiv 2022
    Insert
    PfV
    Use
    CRISPR
    Tags
    Sapphire
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    203660-plasmid-map-sequence-id-402488

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  13. pFETCh_DNMT3B

    Plasmid
    #72365
    Purpose
    Donor vector for 3' FLAG tag of human DNMT3B
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    DNMT3B Homology Arms (DNMT3B Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72365-plasmid-map-sequence-id-132115

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  14. HR_G3BP1-V5-APEX2-GFP

    Plasmid
    #105284
    Purpose
    homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus
    Depositor
    Eugene Yeo
    Article
    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do
    Insert
    G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 soybean, Human)
    Use
    Human homologous recombination donor
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    105284-plasmid-map-sequence-id-229022

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  15. pDONR223-ZNF804B

    Plasmid
    #88756
    Purpose
    Donor Vector containing ZNF804B transcription factor, part of the Human TFome Collection
    Depositor
    Marc Vidal
    Article
    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.
    Insert
    ZNF804B (ZNF804B Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    Last nucleotide of stop codon removed to allow fo…
    Promoter
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only

  18. pDONR221-ETV3

    Plasmid
    #88804
    Purpose
    Donor Vector containing ETV3 transcription factor, part of the Human TFome Collection
    Depositor
    Marc Vidal
    Article
    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.
    Insert
    ETV3 (ETV3 Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    Last nucleotide of stop codon removed to allow fo…
    Promoter
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    88804-plasmid-map-sequence-id-410021

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Showing: 1481 - 1500 of 6257 results