Addgene

We narrowed to 21,291 results for: crispr

Showing: 1481 - 1500 of 21291 results

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gRNA-eGFP-Reporter

Plasmid

#60719

Purpose

Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 and mouse U6

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pRB1017

Plasmid

#59936

Purpose

empty vector for gRNA cloning

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Type

Empty backbone

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAN-NOR

Plasmid

#62306

Purpose

synthetic circuit: NOR gate

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A2NT sgRNA
A2NT sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PPhlF and pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A2NT

Plasmid

#62257

Purpose

expression of A2NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A2NT sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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sgPal7 (p2Tol-U6-sgPal7-HygR)

Plasmid

#71484

Purpose

Expresses palindromic sgRNA for use in self-cloning CRISPR

Depositor

Richard Sherwood

Article

Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

Insert

sgPal7

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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sg_shuttle_RFP657

Plasmid

#134968

Purpose

Lentiviral vector for sgRNA expression

Depositor

Beat Bornhauser

Article

Huang et al Hemasphere. 2020 Sep 30;4(5):e456. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6-sgRNA; EFS-RFP657

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAC1804_pmax-dCasRx-RBM38

Plasmid

#118638

Purpose

Transient transfection; Expresses dCasRx-RBM38; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx-RBM38

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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p(bhsp68-Cas9)

Plasmid

#65959

Purpose

Tribolium basal heat shock promoter driving Cas9

Depositor

Michalis Averof

Article

Gilles et al Development. 2015 Jul 9. pii: dev.125054

Insert

Cas9

Use

CRISPR

Tags

nuclear localization sequence

Expression

Insect

Mutation

Promoter

bhsp

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMA-MsgRNA-EGFP

Plasmid

#80794

Purpose

For insertion of gRNA array containing 11-30 gRNA modules

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos9_AmpR

Plasmid

#119152

Purpose

Encodes sgRNA targeting position 9 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 9 in pColE1_70a_deGFP_KanR

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCD227

Plasmid

#113318

Purpose

AraC-pBAD-dCas9, MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Tags

Expression

Bacterial

Mutation

R93A

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAC1806_pmax-FKBP-dCasRx

Plasmid

#118639

Purpose

Transient transfection; Expresses FKBP-dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

FKBP-dCasRx

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pH1v1

Plasmid

#60244

Purpose

gRNA expression by the H1 promoter

Depositor

Donald Zack

Article

Ranganathan et al Nat Commun. 2014 Aug 8;5:4516. doi: 10.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

H1 promoter

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pAC1803_pmax-RBM38-dCasRx

Plasmid

#118637

Purpose

Transient transfection; Expresses RBM38-dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBM38-dCasRx

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAL-pgi_NT

Plasmid

#74067

Purpose

pAL374 plasmid carrying the pgi (NT) sgRNA, targeting the non-template strand of pgi, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pgi (NT)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ptac

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAL-pyk_NT

Plasmid

#74072

Purpose

pAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pyk (NT)

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

ptac

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAC1807_pmax-dCasRx-FKBP

Plasmid

#118640

Purpose

Transient transfection; Expresses dCasRx-FKBP; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx-FKBP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMZ283

Plasmid

#52224

Purpose

Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments).

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Insert

rrk1-sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

rrk1

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCR1002

Plasmid

#71820

Purpose

Express Streptococcus pyogenes Cas9

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_10xHis-MBP-TEV-SPyCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pAL-pck_NT

Plasmid

#74069

Purpose

pAL374 plasmid carrying the pck (NT) sgRNA, targeting the non-template strand of pck, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA pck (NT)

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ptac

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only