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Purpose(Empty Backbone) empty vector for gRNA cloning
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 59936 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRB1017
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Vector typeWorm Expression, CRISPR
- Promoter U6
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRB1017 was a gift from Andrew Fire (Addgene plasmid # 59936 ; http://n2t.net/addgene:59936 ; RRID:Addgene_59936) -
For your References section:
Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans. Arribere JA, Bell RT, Fu BX, Artiles KL, Hartman PS, Fire AZ. Genetics. 2014 Aug 26. pii: genetics.114.169730. 10.1534/genetics.114.169730 PubMed 25161212