Showing: 1481 - 1500 of 3763 results
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Plasmid#207745PurposeExpression of the Cre recombinase linked to a mScarlet fluorescent markerDepositorArticleInsertCre-PGK-mScarlet
UseCre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pEF1α-Cre-2A-mTagBFP2
Plasmid#207746PurposeExpression of the Cre recombinase linked to a mTagBFP2 fluorescent markerDepositorArticleInsertCre-PGK-mTagBFP2
UseCre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pEF1α-Cre-2A-miRFP670nano3
Plasmid#207747PurposeExpression of the Cre recombinase linked to a miRFP670nano3 fluorescent markerDepositorArticleInsertCre-PGK-miRFP670nano3
UseCre/LoxTagsExpressionMammalianMutationPromoterEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pBTK1050
Plasmid#209540PurposeBTK Type 1 homology arm for Golden Gate assemblyDepositorInsertUpstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK1054
Plasmid#209544PurposeBTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome at a neutral siteDepositorInsertRecombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBTK1061
Plasmid#209550PurposeBTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome in place of the clpA geneDepositorInsertE2-Crimson cassette flanked with homology arms for recombination into the S. alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28a-mScarlet-TEV-Strep-His
Plasmid#210823PurposeBacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent proteinDepositorInsertmScarlet
UseTagsTwinStrep, 10xHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mTagBFP2-TEV-Strep-His
Plasmid#210824PurposeBacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent proteinDepositorInsertmTagBFP2
UseTagsTwinStrep, 10xHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-V314D-TSF
Plasmid#210839PurposeMammalian expression of FIP200 NTD mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsExpressionMammalianMutationV314DPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-R326D-TSF
Plasmid#210840PurposeMammalian expression of FIP200 NTD mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsExpressionMammalianMutationR326DPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-R334D-TSF
Plasmid#210841PurposeMammalian expression of FIP200 NTD mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsExpressionMammalianMutationR334DPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-V44D-TSF
Plasmid#210843PurposeMammalian expression of FIP200 NTD mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsExpressionMammalianMutationV44DPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCDH1-CMV-FIP200-mCherry-Hygro
Plasmid#210852PurposeLentiviral expression vector encoding FIP200-mCherryDepositorInsertFIP200 (RB1CC1 Human)
UseLentiviralTagsmCherryExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCI AscI MR1 res
Plasmid#214752Purposeexpresses human MR1 resistant to CRISPR editing with a specific sgRNA in mammalian cellsDepositorInsertMHC class I-related protein 1 (MR1 Human)
UseTagsIRES eGFPExpressionMammalianMutationsilent mutations to prevent editing by CRISPR/Cas…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLV UBC APEX2-3xFLAG-IRES-eGFP
Plasmid#214983PurposeCytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).DepositorInsertAPEX2 (APX2 Glycine max (soybean))
UseLentiviralTags3xFLAGExpressionMutationPromoterUBCAvailabilityAcademic Institutions and Nonprofits only -
pLV UBC hTMEM115-3C-3xHA-P2A-eGFP
Plasmid#214996PurposeStably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.DepositorInsertTMEM115 (TMEM115 Human)
UseLentiviralTags3xHAExpressionMammalianMutationPromoterUBCAvailabilityAcademic Institutions and Nonprofits only -
pLV UBC msTMEM115-3C-3xHA-P2A-eGFP
Plasmid#214997PurposeStably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.DepositorInsertTMEM115 (Tmem115 Mouse)
UseLentiviralTags3xHAExpressionMammalianMutationPromoterUBCAvailabilityAcademic Institutions and Nonprofits only -
hTMEM115-TEV-3xHA-P2A-mScarlet HDR template
Plasmid#214998PurposeEndogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.DepositorInserthTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)
UseHdr templateTags3xHAExpressionMutationPromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template
Plasmid#214999PurposeEndogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.DepositorInserthTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)
UseHdr templateTags3xHAExpressionMutationPromoterNoneAvailabilityAcademic Institutions and Nonprofits only -
Nuclear PancACe
Plasmid#215706PurposeExpresses acetyl-CoA biosensor in the nucleusDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and NLSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 1481 - 1500 of 3763 results