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pET23a-His-hRanGAP1tail
Plasmid#53139PurposeE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tagDepositorInsertRanGAP1 (RANGAP1 Human)
UseTagsHisExpressionBacterialMutationC-terminal fragment (amino acids 398-587)PromoterT7Available sinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/FLHIP1
Plasmid#29587DepositorInsertHuntingtin-interacting protein 1 (HIP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-CMV10-Flag-mMcl-1 KR
Plasmid#32979DepositorInsertMcl-1 (Mcl1 Mouse)
UseTags3XFlagExpressionMammalianMutationAll lys changed to ArgPromoterCMVAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-Flag-mMcl-1 KR
Plasmid#32983DepositorInsertMcl-1 (Mcl1 Mouse)
UseRetroviralTags3XFlagExpressionMammalianMutationAll Lys to ArgPromoterLTRAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVRc33_375
Plasmid#49748DepositorInsertAS33_375
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
9X NFAT-apha-MHC-Luc
Plasmid#51941Purpose9 copies of NFAT sites in minimal alphaMHC-pGL-3DepositorInserts9x NFAT binding site
alpha-MHC
UseLuciferaseTagsluciferaseExpressionMutationPromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-fLuc
Plasmid#40589DepositorInserthGFAP promoter (GFAP Human)
UseLuciferaseTagsFirefly luciferaseExpressionMutationContains human GFAP promoter fragment (-2163 to +…PromoterGFAP promoterAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
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CMV_Npu-BE3.9max C-terminal
Plasmid#137172PurposeExpresses the C-terminal split-intein fragment of BE3.9max from the CMV promoterDepositorInsertNpu-BE3.9max_C-terminal
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-ABEmax C-terminal
Plasmid#137174PurposeExpresses the C-terminal split-intein fragment of ABEmax from the CMV promoterDepositorInsertNpu-ABEmax_C-terminal
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGIP*35
Plasmid#26776PurposeEnables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targetingDepositorInsertGFP*35
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/HIP1/ΔC
Plasmid#31250DepositorInsertHuntingtin-interacting protein 1 (HIP1 Human)
UseTagsExpressionBacterialMutationDeltion of nucleotides 1336-2513 1386bp C to T…PromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-IV
Plasmid#53440PurposeDerivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IVDepositorInsertagrBD locus (with D29Y mutation in agrD)
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationD to Y mutation of residue 29 of the agrD gene (c…PromoterT7-lacAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-ABE N-terminal
Plasmid#137177PurposeAAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoterDepositorInsertv5 AAV-ABE N-terminal
UseAAVTagsExpressionMutationCas9 D10APromoterCbhAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGIP
Plasmid#26777PurposeLentiviral vector for stable integration of GFP expression cassette with puromycin selection.DepositorInsertEF1a-GFP-IRES-Puro (gfp )
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSynI-rtTAV16
Plasmid#102367PurposeAAV vector to drive TET-ON activator expression from human synapsin I promoterDepositorInsertrtTAV16
UseAAVTagsExpressionMammalianMutationrtTA variant reported in Gene Therapy (2006) 13, …Promoterhuman synapsin I promoterAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_WT
Plasmid#162579PurposeExpresses norovirus GI.1 VP1 protein in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1_HBB_N
Plasmid#87847PurposepCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNADepositorInsertHomo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV_Npu-BE3.9max N-terminal
Plasmid#137171PurposeExpresses the N-terminal split-intein fragment of BE3.9max from the CMV promoterDepositorInsertNpu-BE3.9max_N-terminal
UseTagsExpressionMammalianMutationCas9 D10APromoterCMVAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only
