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Plasmid#11524DepositorInsertprostate-specific membrane antigen (FOLH1 Human)
UseTagsHisExpressionInsectMutationThere is a short N-terminal truncation of this pr…PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
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p3E-FRT-KanR-FRT pA
Plasmid#82601Purpose3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selectionDepositorInsertFRT-Kan-FRT
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-DSCP-Gateway
Plasmid#71507PurposeflySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the DSCP core promoterDepositorTypeEmpty backboneUseFlystarr-seq validation vectorTagsluciferaseExpressionInsectMutationPromoterAvailable sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-KA
Plasmid#64981PurposeExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108APromoterAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA
Plasmid#64973PurposeExpresses the wild type of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59PromoterT7 promoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-Rac1-Q61L
Plasmid#214292PurposeExpress constitutively active Rac1 fused with MBPDepositorInsertConstitutively active Rac1 (RAC1 Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationRac1-Q61LPromoterTacAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-Rac1-T17N
Plasmid#214293PurposeExpress dominant negative Rac1 fused with MBPDepositorInsertDominant negative Rac1 (RAC1 Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationRac1-T17NPromoterTacAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYGS Q16C
Plasmid#217674PurposeFor expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experimentDepositorInsertCRYGS (CRYGS Human)
UseTags6XHis and SUMOExpressionBacterialMutationGlutamine 16 changed to CysteinePromoterT7Available sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-RBCS-tagRFP
Plasmid#213868PurposeBinary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)DepositorInsertRIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTS2351_Tier3(SB)-Zeo
Plasmid#169648PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a ZeoR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-ZeoR-pA-3'ITR)DepositorInsertPRPBSA-driven ZeoR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK278
Plasmid#71134PurposeExpression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to asparagine; additional N-…PromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK130
Plasmid#71129PurposeExpression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsHis6ExpressionBacterialMutationchanged valine-319 to phenylalaninePromoterT7Available sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK173
Plasmid#71831PurposeCloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE, and purK genesDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK320
Plasmid#71144PurposeExpression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to serine; additional N-term…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK319
Plasmid#71143PurposeExpression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to alanine; additional N-ter…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK318
Plasmid#71142PurposeExpression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to glutamine; additional N-t…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK315
Plasmid#71141PurposeExpression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to glycine; additional N-ter…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK314
Plasmid#71140PurposeExpression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-89 to valine; additional N-term…PromoterT7Available sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK174
Plasmid#71835PurposeExpression of Acetobacter aceti 1023 PurE and PurK in E. coliDepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationadditional N-terminal methionine encoded by this …PromoterT7 and endogenousAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only