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Bacterial Strain#37167DepositorBacterial ResistanceAmpicillin and kanamycinAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only
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pRelA*
Plasmid#175590PurposeTet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.DepositorInsertCatalytic domain of E.coli relA gene.
UseTagsFused with a flexible GS linker to mVenusExpressionBacterialMutationOnly the catalytic domain of the enzyme is used (…PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
AA009
Plasmid#216037PurposeFragmid fragment: (C' terminus) Nucleoplasmin NLSDepositorInsertNpNLS_v1.1; 3xHA_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA295
Plasmid#215986PurposeFragmid fragment: (N' terminus) SV40 Nuclear Localization SignalDepositorHas ServiceCloning Grade DNAInsertStart; SV40NLS_v1.19
UseFragmentTagsExpressionMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA014
Plasmid#216057PurposeFragmid fragment: (2A Selection) Puromycin resistanceDepositorInsertP2A_v1.1; PuroR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA163
Plasmid#216061PurposeFragmid fragment: (2A Selection) Stop onlyDepositorInsertStop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA065
Plasmid#215965PurposeFragmid fragment: (Pol 2 promoter) human EF1a promoterDepositorHas ServiceCloning Grade DNAInsertEF1a_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA082
Plasmid#215997PurposeFragmid fragment: (Selection) fluorescent reporterDepositorInsertStart; mTagBFP2_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA015
Plasmid#216058PurposeFragmid fragment: (2A Selection) Puromycin resistance, T2A, EGFPDepositorInsertP2A_v1.1; PuroR_v1.1; T2A_v1.2; EGFP_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA176
Plasmid#216063PurposeFragmid fragment: (2A Selection) mCherryDepositorInsertP2A_v1.1; mCherry_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA010
Plasmid#216038PurposeFragmid fragment: (C' terminus) for C>T base editingDepositorInsertUGI_v1.1; NpNLS_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA041
Plasmid#215963PurposeFragmid fragment: (Pol 2 promoter) human core (short) EF1a promoterDepositorHas ServiceCloning Grade DNAInsertEFS_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA013
Plasmid#215975PurposeFragmid fragment: (N' terminus) for A>G base editingDepositorHas ServiceCloning Grade DNAInsertStart; BPNLS_v1.1; ABE8e_v1.1; Linker_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-RiboJ-LacZa-T7
Plasmid#173224PurposepFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systemsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
His-DNPH1
Plasmid#203224PurposeFor protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertDNPH1 (DNPH1 Human)
UseTagsHis tag (with TEV cleavage site)ExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-RiboJ-mScarlet-T7
Plasmid#173227PurposeEncodes mScarlet fluorescent protein. To be used in cell-free systemDepositorInsertmScarlet
UseCell-free systemTagsExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
AA019
Plasmid#216039PurposeFragmid fragment: (C' terminus) VP64; NpNLSDepositorInsertVP64_v2.1; NpNLS_v1.1
UseFragmentTagsExpressionMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-A-ccdB-G-Z
Plasmid#218892Purposegolden gate cloningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-W-A-ccdB-G-X
Plasmid#218891Purposegolden gate cloningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA335
Plasmid#216069PurposeFragmid fragment: (2A Selection) Stop; IRES2; Start; HygroR; StopDepositorInsertStop; IRES2_v1; Start; HygroR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only