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Plasmid#219841Purposeequimolar expression of yemiRFP670 and mTurquoise2 in budding yeastDepositorInsertyemiRFP670-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EF1a-ArgiNLS-miRFP670
Plasmid#220600PurposeConstitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.DepositorInsertmiRFP670
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKSR1-CA3-mRFP1 (C-KSR)
Plasmid#217754PurposeFluorescent reporter for ceramide (mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseTagsmRFPExpressionMammalianMutationCA3 domain aa 317-400 plus vector-based linker LE…PromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKSR1-CA3-GS-mRFP1 (C-KSR-GS)
Plasmid#217758PurposeFluorescent reporter for ceramide (mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseTagsmRFPExpressionMammalianMutationCA3 domain aa 317-400 with GGSSGGGGA linkerPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAD-5PSD95-EGFP-SynPhRFP
Plasmid#217979PurposeG-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFPDepositorUseG-deleted rabiesTagsEGFP and RFPExpressionMutationPromoterNoneAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304_miRFP670nano-P2A-ERM-gCam6f
Plasmid#214927PurposeExpress miRFP670nano P2A ERM-gCaMP6f (fluorescent proteins and genetic calcium sensor, gCaMP6f for normalizing factor and sensing calcium ions at cytosol exposed to ERM, respectively)DepositorInsertmiRFP670nano-P2A-ERM-gCam6f
UseLentiviralTagsExpressionMammalianMutationPromoterCMVpAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Golgi-FAST-IRES-iRFP670
Plasmid#216345PurposeFor testing localization of pFAST and iRFP670 proteins to Golgi complexDepositorInsertST6Gal1_pFAST-IRES-Sys1_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Peroxisomes-FAST-IRES-iRFP670
Plasmid#216347PurposeFor testing localization of pFAST and iRFP670 proteins to peroxisomesDepositorInsertpFAST_SKL-IRES-iRFP670_PXMP2
UseTagsmyc for FAST protein onlyExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mito-FAST-IRES-iRFP670
Plasmid#216346PurposeFor testing localization of pFAST and iRFP670 proteins to mitochondriaDepositorInsertCox8_pFAST-IRES-Tomm20_iRFP670
UseTagsmyc for FAST and iRF670 proteinsExpressionMammalianMutationn/aPromoterCMVAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL6-H2B-iRFP720-bla
Plasmid#189991PurposeLentivirial plasmid for nuclear iRFP-720 expression with blasticidin resistanceDepositorInsertHuman histone H2B
UseLentiviralTagsiRFP720ExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIhyg-iRFP670-P2Av3-RGECO1.0
Plasmid#184713PurposeCalcium biosensorDepositorInsertGCaMP6s
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-4gRNA-GBX2-RFP
Plasmid#192288PurposeExpresses RFP and four sgRNAs against GBX2DepositorInsertRFP and four sgRNA GBX2 (GBX2 Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-sTagRFP-TUBA1B
Plasmid#207765PurposeDonor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorArticleInsertTUBA1B Homology Arms flanking a sTagRFP Tag (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Puro-sTagRFP-TUBA1B
Plasmid#207769PurposeDonor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorArticleInsertTUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-PRLSS-NbGFP_miRFP670nano3-KDEL
Plasmid#209718PurposeMammalian expression of NbGFP-miRFP670nano3 localized to endoplasmic reticulumDepositorInsertPRLSS-NbGFP_miRFP670nano3-KDEL
UseTagsPRLSSExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-SOX2-T2A-miRFP670
Plasmid#187375PurposeExpresses human SOX2 and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertSOX2 (SOX2 Human)
UseTagsT2A-miRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW emiRFP2-T2A-mtq2
Plasmid#205497PurposeExpression of emiRFP2-T2A-mtq2 in yeastDepositorInsertemiRFP2-T2A-mtq2
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW miRFP680-T2A-mtq2
Plasmid#205502PurposeExpression of miRFP680-T2A-mtq2 in yeastDepositorInsertmiRFP680-T2A-mtq2
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW mfRFP-T2A-mtq2
Plasmid#205494PurposeExpression of mfRFP-T2A-mtq2 in yeastDepositorInsertmfRFP-T2A-mtq2
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yoTagRFP657-T2A-mtq2
Plasmid#205505PurposeExpression of yoTagRFP657-T2A-mtq2 in yeastDepositorInsertyoTagRFP657-T2A-mtq2
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only