Addgene

We narrowed to 5,521 results for: #16

Showing: 1461 - 1480 of 5521 results

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pACEBac1-PARP2-Flag-His-WT

Plasmid

#111574

Purpose

Expresses full length PARP2 in Sf9 (insect) cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP2 (PARP2 Human)

Use

Tags

C-terminal 6xHis and C-terminal Flag

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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β3-integrin-YFP

Plasmid

#26653

Depositor

Jonathan Jones

Article

Tsuruta et al FASEB J. 2002 Jun . 16(8):866-8.

Insert

β3-integrin (ITGB3 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pLv-CMV-MyoD-ER(T)

Plasmid

#26809

Depositor

Jeffrey Chamberlain

Article

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

MyoD-ER(T) (Myod1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only
pET28C-SARS-CoV-2-Nucleocapsid

Plasmid

#204402

Purpose

E. coli expression of full-length SARS-CoV-2 nucleocapsid

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

SARS-CoV-2 nucleocapsid (N SARS-CoV-2)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag p38 beta2

Plasmid

#20355

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 beta2 (MAPK11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pHH0103_PPP1CA_Metallophos

Plasmid

#109934

Purpose

Protein expression and purification of PPP1CA_Metallophos

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PPP1CA_Metallophos

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDIV303

Plasmid

#204978

Purpose

AMA1 plasmid with Aspergillus optimized Mad7 and amdS selection marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Inserts

Mad7
amdS

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

two silent mutations (nt177T>C, nt879C>T)

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-EPLIN alpha

Plasmid

#40928

Depositor

Scott Maul, Elizabeth Luna

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
pLOX-CW-CRE

Plasmid

#12238

Depositor

Didier Trono

Article

Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

Insert

CRE

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

CMV promoter is upstream of nls-CRE. LoxP in 3&#x…

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pJ3H PTP-1B

Plasmid

#8601

Depositor

Ben Neel

Article

Venable et al J Biol Chem. 2000 Jun 16. 275(24):18318-

Insert

PTP-1B (PTPN1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K103R

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pCDNA5_FRT_TO_TurboID-mCherry-Cry2

Plasmid

#166504

Purpose

Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.

Depositor

Angelos Constantinou

Article

Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2

Insert

None

Use

Tags

TurboID and mCherry-CRY2

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

Plasmid

#175523

Purpose

Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag11 and Tomm20-HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_PLK1_Pkinase

Plasmid

#109933

Purpose

Protein expression and purification of PLK1_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PLK1_Pkinase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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mpx:UtrCH-GFP

Plasmid

#45247

Depositor

Anna Huttenlocher

Article

Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

Insert

utrophin (UTRN Human)

Use

Zebrafish expression

Tags

EGFP

Expression

Mutation

contains amino acids 1-261 of NP_009055.2; Q236R

Promoter

mpx

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pMP71-tCD19-mGFP

Plasmid

#174609

Purpose

expresses palmitoylated GFP and extracellular and transmembrane domains of murine CD19

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

expresses the extracellular and transmembrane domain of murine CD19 and palmitoylated GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-PTEN

Plasmid

#110181

Purpose

Expression of PTEN tagged with EGFP

Depositor

Jose Esteban

Article

Jurado et al EMBO J. 2010 Aug 18;29(16):2827-40. doi:

Insert

PTEN (Pten Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_ACTG1_Actin

Plasmid

#109989

Purpose

Protein expression and purification of ACTG1_Actin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ACTG1_Actin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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FU- dio Teal-gephyrin - W

Plasmid

#73918

Purpose

To express the inhibitory scaffold gephyrin tagged to teal fluorescent protein. Expression is Cre dependant. Plasmid can also be used to make lentivirus.

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

Teal-gephyrin

Use

Lentiviral

Tags

Teal

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-DDX4LCD

Plasmid

#204408

Purpose

E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

DDX4(1-236) (DDX4 Human)

Use

Tags

6x-His; mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMP71-tCD19-SIINFEKL

Plasmid

#174601

Purpose

expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the SIINFEKL epitope

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

membrane bound CD19, SIINFEKL (ovalbumin epitope)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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