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pFBD-ESCRT 0
Plasmid#21499DepositorInsertpFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Human, Mouse)
UseTagsGST and His-MBPExpressionInsectMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG_N2N3-HIS-ybbr
Plasmid#101238PurposeSdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertSdrG_N2N3
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAMS366-4xCDRE-GFP-PEST
Plasmid#138658Purposedestabilized GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiaeDepositorInsert4xCDRE-CYC1(PM)-yEGFP3-PEST
UseYeast reporter plasmidTagsExpressionMutationPromoterCYC1Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-Q
Plasmid#64980PurposeExpresses the E108Q mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E108QPromoterAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE
Plasmid#20294PurposeLentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to SerinePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGGF002
Plasmid#48843PurposeProvides a BASTA resistance cassette as GreenGate module.DepositorInsertp35S::BastaR::t35S
UseGolden gate compatible cloning vectorTagsExpressionMutationinternal BsaI recognition site in 35S promoter re…PromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE
Plasmid#20293PurposeLentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to AlaninePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE
Plasmid#20295PurposeLentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to ThreoninePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
297_pETcon_SARS2_Omicron-BA286
Plasmid#222231Purposeyeast surface display of the SARS-CoV-2 BA.2.86 variant RBDDepositorInsertSARS-CoV-2 BA.2.86 RBD (S SARS-CoV-2 virus, Budding Yeast)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1-FKBPflex-OSBP-siRNAr
Plasmid#220066PurposesiRNA resistant ORP5 attcahed with anFKBP and a mCherry fluorophoreDepositorInsertOSBPL5 siRNA resistant (OSBPL5 Human)
UseTagsExpressionMammalianMutationsiRNA resistantPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYBA4
Plasmid#217671PurposeFor expression of SUMO tagged human beta A4 crystallinDepositorInsertCRYBA4 (CRYBA4 Human)
UseTags6XHis and Smt3ExpressionBacterialMutationPromoterT7Available sinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK324
Plasmid#71838PurposeCloned region contains Acetobacter aceti 1023 purE geneDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK340
Plasmid#71837PurposeCloned region contains Acetobacter aceti 1023 purE(H59N) geneDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparaginePromoterlacAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK346
Plasmid#71836PurposeCloned region contains Acetobacter aceti 1023 purE(H59D) geneDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges histidine-59 to aspartatePromoterlacAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-A
Plasmid#64979PurposeExpresses the E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E108APromoterAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-K
Plasmid#64978PurposeExpresses the E105K mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105KPromoterAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG_N2N3-B1-B2-HIS-ybbr
Plasmid#117979PurposeSdrG N2 and N3, B1 and B2 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertSdrG_N2N3-B1-B2
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG_N2N3-B1-HIS-ybbr
Plasmid#117980PurposeSdrG N2 and N3, B1 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertSdrG_N2N3-B1
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrD_B1-DK
Plasmid#117983PurposeSdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B2
UseTagsHIS, sortase, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only
