We narrowed to 7,311 results for: ski

1. pCXLE-dCas9VPPH-T2A-GFP-shP53

   Plasmid

   #102897

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9VPPH-T2A-GFP-shP53
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-SARS2-Spike-D614G

   Plasmid

   #166850

   Purpose

   For expression of SARS-CoV-2 Spike D614G in mammalian cells (C9 tagged at C-term)

   Depositor

   Neville Sanjana

   Article

   Daniloski et al Elife. 2021 Feb 11;10. pii: 65365. doi:

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2 virus)
   

   Use

   Tags

   C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCellFree_G03 TFAM

   Plasmid

   #67064

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TFAM (TFAM Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   S115C

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX

   Plasmid

   #188993

   Purpose

   A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   PLD
   

   Use

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   D201G, A258T, K327R, G328S, G381V, G429D, T450A

   Promoter

   CMV

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJEP301-pAAV-CMV-MCS3-pA

   Plasmid

   #113676

   Purpose

   CMV driven Multi Cloning Site-3

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   N/A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Cytomegalo Virus(CMV)

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX-GP-2-DNMT3A PWWP

   Plasmid

   #59696

   Purpose

   Contains histone modification interacting domain (DNMT3A PWWP)

   Depositor

   Albert Jeltsch

   Article

   Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

   Insert

   DNMT3A PWWP (Dnmt3a Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-GP-2-CBX7 Chromo

   Plasmid

   #59695

   Purpose

   Contains histone modification interacting domain (CBX7 Chromo)

   Depositor

   Albert Jeltsch

   Article

   Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

   Insert

   CBX7 Chromo (CBX7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCXLE-dCas9VPP300-T2A-GFP-shP53

   Plasmid

   #102896

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9VPP300-T2A-GFP-shP53
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJEP306-pAAV-EFS-MCS3-pA

   Plasmid

   #113682

   Purpose

   EFS driven Multi Cloning Site-3.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   N/A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Cytomegalo Virus(CMV)

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCellFree_G03 EBNA1BP2

    Plasmid

    #67072

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    EBNA1BP2 EBNA1BP2 EBNA1 binding protein 2 (EBNA1BP2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCellFree_G03 GTF2E1

    Plasmid

    #67089

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2E1 (GTF2E1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCellFree_G03 FOXM

    Plasmid

    #67118

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    FOXM (FOXM1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-hSyn-hM3D(Gq)::RAM-d2tTA

    Plasmid

    #189627

    Purpose

    Expresses hM3D(Gq) under the hSyn promoter, and d2tTA under the pRAM (synthetic Fos) promoter. Plasmid 1 for recording Fos expression.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    hM3D(Gq)
    d2tTA
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn and pRAM

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBABEpuro-ARAF_S214C

    Plasmid

    #51077

    Purpose

    mammalian expression of ARAF S214C

    Depositor

    Matthew Meyerson

    Article

    Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

    Insert

    ARAF (ARAF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S214C

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PHR-GFP

    Plasmid

    #183926

    Purpose

    Optogenetic PHR domain coupled to GFP; binds to CIBN upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    PHR-GFP (CRY2 Mustard Weed, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLEX-FHH-MAPKAP1RBD/Triple Mutant

    Plasmid

    #120711

    Purpose

    Expresses Flag-HA-6xHIS-MAPKAP1 RBD Triple Mutant fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MAPKAP1 RBD
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Amino acids 279-353, K307A, K310A, R312L

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pscALPS-SIV-AGM-TAN1 vpr

    Plasmid

    #115819

    Purpose

    Encodes codon optimized 3xFLAG-SIVAGM Vpr (TAN1) (U58991) with repaired premature stop codon and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVAGM Vpr (TAN1)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19 PKC epsilon

    Plasmid

    #8442

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Mar 25;268(9):6090-6.

    Insert

    Mouse Protein Kinase C epsilon (Prkce Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pLTR PKC zeta

    Plasmid

    #8421

    Depositor

    Frederic Mushinski

    Article

    Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

    Insert

    Mouse Protein Kinase C zeta (Prkcz Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pCellFree_G03 GTF2A1

    Plasmid

    #67076

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2A1 (GTF2A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only