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Plasmid#111977PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R775A mutation. LethalDepositorInsertHsh155 R775A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationR775APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R778A 3xFLAG
Plasmid#111979PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R778A mutation. LethalDepositorInsertHsh155 R778A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationR778APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 AAAA
Plasmid#87600PurposeEncodes Prp5 AAAA for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationD136A, P137A, L138A, D139APromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 TAG
Plasmid#87603PurposeEncodes Prp5 SAT motif to TAG for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationS446T, T448GPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 K335R
Plasmid#87299PurposeEncodes Y2H Gal4 activation domain for Hsh155 K335RDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 AD and HAExpressionBacterial and YeastMutationK335RPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-CLF1
Plasmid#87548PurposeEncodes Y2H Gal4 DNA binding domain for Clf1DepositorInsertCLF1 (CLF1 Budding Yeast)
UseTagsGAL4 BD and MycExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 D450G
Plasmid#87303PurposeEncodes Y2H Gal4 activation domain for Hsh155 D450GDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 ADExpressionBacterial and YeastMutationD450GPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 P369K
Plasmid#87302PurposeEncodes Y2H Gal4 activation domain for Hsh155 P369KDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 ADExpressionBacterial and YeastMutationP369KPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 H331Q
Plasmid#87296PurposeEncodes Y2H Gal4 activation domain for Hsh155 H331QDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 ADExpressionBacterial and YeastMutationH331QPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 H331D
Plasmid#87295PurposeEncodes Y2H Gal4 activation domain for Hsh155 H331DDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 ADExpressionBacterial and YeastMutationH331DPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 N295D
Plasmid#87294PurposeEncodes Y2H Gal4 activation domain for Hsh155 N295DDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 ADExpressionBacterial and YeastMutationN295DPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 R294L
Plasmid#87293PurposeEncodes Y2H Gal4 activation domain for Hsh155 R294LDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 ADExpressionBacterial and YeastMutationR294LPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 N295D/D450G
Plasmid#87287PurposeEncodes Hsh155 N295D D450G for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationN295D & D450GPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 R294L/H331D
Plasmid#87282PurposeEncodes Hsh155 R294L H331D for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationR294L & H331DPromoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 N295D/H331D
Plasmid#87285PurposeEncodes Hsh155 N295D H331D for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationN295D & H331DPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 P369E
Plasmid#87301PurposeEncodes Y2H Gal4 activation domain for Hsh155 P369EDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 AD and HAExpressionBacterial and YeastMutationP369EPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 K335T
Plasmid#87300PurposeEncodes Y2H Gal4 activation domain for Hsh155 K335TDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 AD and HAExpressionBacterial and YeastMutationK335TPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 K335N
Plasmid#87298PurposeEncodes Y2H Gal4 activation domain for Hsh155 K335NDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 AD and HAExpressionBacterial and YeastMutationK335NPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 K335E
Plasmid#87297PurposeEncodes Y2H Gal4 activation domain for Hsh155 K335EDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 AD and HAExpressionBacterial and YeastMutationK335EPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGADT7 Hsh155 R294C
Plasmid#87292PurposeEncodes Y2H Gal4 activation domain for Hsh155 R294CDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsGAL4 AD and HAExpressionBacterial and YeastMutationR294CPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only