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pLX-EF1a_mCherry
Plasmid#221482PurposemCherry lentiviral overexpressionDepositorInsertmCherry
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE 3xHA-MKK6-EE puro
Plasmid#216791PurposeMammalian expression of constitutively active human MKK6DepositorInsertMKK6 (MAP2K6 Human)
UseRetroviralTags3x-HAExpressionMammalianMutationS207E and T211E, constitutively activePromoterLTRAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMta2-EosHalo-TAFiP
Plasmid#140959PurposeTargeting construct to make Mta2-Eos-Halo-Tev-Avi-3xFlag fusionDepositorInsertmetastasis-associated gene family, member 2 (Mta2 Mouse, Synthetic)
UseMouse TargetingTags3xFLAG, Avi, Eos, Halo, and TEVExpressionMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-dsRed_IDG-K
Plasmid#135260PurposeGateway destination clone of dsRed (as control) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-dsRed
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
ELL1 CtoA
Plasmid#127279PurposeTesting if ELL1 C to A mutation knock out the action on Myc as it does in humanDepositorInsertELL1 (NM_007924 plus CEYLHNK to AEYLHNK) (ELL Mouse)
UseTagsTwin strep-tagExpressionMammalianMutationCEYLHNK to AEYLHNK by Q5 mutagenesisPromoterEF1aAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/LL5BIP-GFP
Plasmid#112831Purposetransient/stable overexpressionDepositorInsertLL5BIP (2410002F23Rik Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-mRIPK3 AAAA/D143N-Flag/cHA-pTRIPZ vector
Plasmid#73706Purposelentiviral expression of mRIPK3 mutantDepositorInsertRIPK3 (Ripk3 Mouse)
UseLentiviralTagsFlag and HAExpressionMammalianMutation448-AAAA-451 and D143NPromoterCMV/tetAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYJA5
Plasmid#217778PurposeThe empty vector for quadruple sgRNA cloningDepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsPuromycin resistance gene, T2A, and TagBFPExpressionMammalianMutationPromoterPGK, CMV, U6Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-mgp100-PuroR [M1G]
Plasmid#171802PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-mgp100-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE-EGFP-FLAG-PSMD2
Plasmid#161917PurposeExpresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.DepositorInsert26S proteasome non-ATPase regulatory subunit 2 (PSMD2 Human)
UseTagsGFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) SDC3
Plasmid#107501PurposeExpresses SDC3, puromycin resistantDepositorInsertSDC3 (codon optimized) (SDC3 Human)
UseLentiviralTagsExpressionMammalianMutationCodon optimized sequencePromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)
Plasmid#207357PurposeLentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertL227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) MXRA7
Plasmid#107500PurposeExpresses MXRA7, puromycin resistantDepositorInsertMXRA7 (codon optimized) (MXRA7 Human)
UseLentiviralTagsExpressionMammalianMutationCodon optimized sequencePromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) VOPP1
Plasmid#107507Purposeexpressing VOPP1, puromycin resistantDepositorInsertVOPP1 (VOPP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-hgp100-PuroR [M1G]
Plasmid#171801PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-hgp100-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) RALB
Plasmid#107506PurposeExpressing RALB, puromycin resistantDepositorInsertRALB (RALB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) FITM2
Plasmid#107504PurposeExpresses FITM2, puromycin resistantDepositorInsertFITM2 (codon optimized)
UseLentiviralTagsExpressionMammalianMutationCodon optimized sequencePromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mScarlet-F-Ova-PuroR [M1G]
Plasmid#171813PurposeUniversal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]DepositorInsertmScarlet-F-Ova-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)
Plasmid#207356PurposeLentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertN1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only
