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Plasmid#136069PurposeAcceptor plasmid for even level position 3, lacZ blue-white screeningDepositorInsertlacZ
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCsE
Plasmid#136070PurposeAcceptor plasmid for even level position 4, lacZ blue-white screeningDepositorInsertlacZ
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPIPL12-FnCas12a
Plasmid#199563PurposeTetracycline-Inducible Fncas12a gene expression in E. coli and clostridiaDepositorInsertFncas12a
UseCRISPR and Synthetic Biology; Tetracycline induci…TagsExpressionBacterialMutationPromoterCOtetR-PIPL12Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1_WT_OCT4
Plasmid#40633DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsGST, TEV cleavage, and Thrombin cleavageExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJCC_039 FnCas12a
Plasmid#171666PurposeFor bacterial expression of FnCas12a with a C-terminal sorting motif and His tagDepositorInsertFnCas12a
UseTags6X His and sortase A sorting motifExpressionBacterialMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCsA
Plasmid#136067PurposeAcceptor plasmid for even level position 1, lacZ blue-white screeningDepositorInsertlacZ
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJYS1Peftu
Plasmid#85546PurposeConstitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoterDepositorInsertsCpf1
recT
UseCRISPR; Shuttle vector corynebacterium glutamicum…TagsExpressionBacterialMutationPromoteretfu and unknownAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
L1_HyR-Ck1
Plasmid#136125PurposeL1 in position 1 (next to LB in L2 construct), for HygRDepositorInsertp5-35S:hptII_3t35S
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAVS1_Puro_hPGK1_EBFP_Donor
Plasmid#178089PurposeHDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertEBFP (T65S/F145Y)
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1Available sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV53-Cas12a
Plasmid#158706PurposeCRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61DepositorInsertFnCpf1
UseCRISPRTagsExpressionBacterialMutationPromoterTetOAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only