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Plasmid#160389PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selectionDepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseCen/arsTagsExpressionYeastMutationPromoterEndogenous ATG8 promoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG35
Plasmid#35123DepositorInsertnatMX3
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAG36
Plasmid#35126DepositorInsertnat1
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_LEU2_I
Plasmid#109060Purposeyeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposesDepositorInsertLEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterLEU2Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
isoform PKC Gamma (JCE692)
Plasmid#89780Purposeyeast expression of mouse PKC Gamma with yeast PKC1 promoter and ADH1 terminatorDepositorInsertPKC Gamma (Prkcg Mouse)
UseTagsExpressionMammalianMutationPromoteryeast PKC1 promoter (-600 to -1)Available sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB5230
Plasmid#215496PurposeA pAde6 backbone integrating plasmid expressing Pil1-mCherry-SpAtg8(1-115) under the inducible promoter 41nmt1DepositorInsertPil1-mCherry-SpAtg8(1-115)
UseTagsExpressionYeastMutationPromoterP41nmt1Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTEF:ATP
Plasmid#92179PurposeHO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.DepositorInsertATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
UseIntegration and expression into yeast.TagsYeast codon optimized mseCFP (donor) and cp173-mV…ExpressionYeastMutationPromoterTEF1pAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-HsAIDSc
Plasmid#60810Purposeto express human AID recoded for yeast codon usageDepositorInsertAID (AICDA Human)
UseTagsMycExpressionYeastMutationcodon optimized for yeastPromoterGAL1Available sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C
Plasmid#60394PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptideDepositorInsertTUB1 (TUB1 Budding Yeast, Synthetic)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable sinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMZ374
Plasmid#59896PurposeCombination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).DepositorInsertsCas9
rrk1:sgRNA
UseCRISPRTagsExpressionYeastMutationSilent muation of the CspCI sitePromoteradh1 and rrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCY 3090-06
Plasmid#36233DepositorInsertURA3
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-mCherry-URA3ExpressionMutationPromoterAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCY 3010-05
Plasmid#36211DepositorInsertLEU2
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-Cerulean-LEU2ExpressionMutationPromoterAvailable sinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCY 3040-05
Plasmid#36220DepositorInsertLEU2
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEGFP-LEU2ExpressionMutationPromoterAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCY 3080-04
Plasmid#36228DepositorInsertTRP1
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEmCitrine-TRP1ExpressionMutationPromoterAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCY 3080-06
Plasmid#36230DepositorInsertURA3
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEmCitrine-URA3ExpressionMutationPromoterAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCY 3080-07
Plasmid#36227DepositorInsertStreptoalloteichus hindustanus bleomycin (Sh ble)
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEmCitrine-ZeocinExpressionMutationPromoterAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCY 3040-04
Plasmid#36219DepositorInsertTRP1
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEGFP-TRP1ExpressionMutationPromoterAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU2-RAD30cmyc
Plasmid#60821Purposeto overexpress yeast polymerase eta in yeastDepositorInsertRAD30 (RAD30 Budding Yeast)
UseTagsc-mycExpressionYeastMutationPromoterGAL1Available sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCY 3120-04
Plasmid#36238DepositorInsertTRP1
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yECitrine-13Myc-TRP1ExpressionMutationPromoterAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only