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GG-EBNA-EEA-5guides-PGK-Puro
Plasmid#102898PurposeEBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.DepositorInsertEEA-5guides-PGK-Puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Gad65P-eGFP-3XNLS-WPRE-pA
Plasmid#183804PurposeAAV vector to express nuclear localized eGFP from a Gad65 promoterDepositorInserteGFP-NLS
UseAAVTagsNLSExpressionMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
GG-EBNA-OSK2M2L1-PP
Plasmid#102902PurposeEBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.DepositorInsertOSK2M2L1-gRNAs-PGK-puro
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHP138 Myc-TRF2-deltaB deltaM
Plasmid#164255Purposefor generation of mammalian cells with inducible expression of dominant negative TRF2 for induction of telomere crisis used together with any PiggyBac transposase plasmidDepositorInsertTRF2
UseTagsMycExpressionMammalianMutationdeletion of amino acids-86 and 496-542PromoterTetracycline inducible promotorAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - wt
Plasmid#65071PurposeRhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoCDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenusPromoterCMV, p10Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-mNeonGreen-CAMSAP3
Plasmid#191322PurposeExpresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flowDepositorInsertmNeonGreen-CAMSAP3 (Camsap3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9GFP-IRES-Neo
Plasmid#102907PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.DepositorInsertDDdCas9GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
NLS-HA-tdMCP-tdTomato
Plasmid#183938Purposetandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tagDepositorInserttdMCP-tdTomato (cp Synthetic)
UseTagsNLS-HAExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CIBN-CAAX
Plasmid#188989PurposeA lentiviral plasmid encoding plasma membrane-tagged CIBNDepositorInsertCIBN (CIB1 Mustard Weed)
UseTagsCAAXExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0013
Plasmid#117549PurposeLevel 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-SETDB1 ts1
Plasmid#115859PurposeSETDB1 knockdownDepositorInsertSETDB1 shRNA (SETDB1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
NLS-dCas9-HA-NLS--GFP-p300
Plasmid#183925Purposep300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFPDepositorInsertdCas9-GFP-p300
UseCRISPRTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-MPHOSPH8 ts2
Plasmid#115875PurposeMPHOSPH8 knockdownDepositorInsertMPHOSPH8 shRNA (MPHOSPH8 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
rTetR-GFP-VP16
Plasmid#183917PurposeVP16 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline additionDepositorInsertrTetR-GFP-VP16 (UL48 Synthetic)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pscALPS gag-gfp/vpx
Plasmid#115808PurposeSFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpxDepositorInsertgag-gfp
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-MPHOSPH8 ts3
Plasmid#115876PurposeMPHOSPH8 knockdownDepositorInsertMPHOSPH8 shRNA (MPHOSPH8 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FKBP-Rab5-Blasticidin
Plasmid#120715PurposeExpresses early endosome-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertRab5a
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutationAmino acids 2-215PromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-PPHLN1 ts1
Plasmid#115877PurposePPHLN1 knockdownDepositorInsertPPHLN1 shRNA (PPHLN1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTH PKC zeta
Plasmid#8414DepositorInsertMouse Protein Kinase C zeta (Prkcz Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only
