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Plasmid#200105DepositorInsertTwist3:cTer-KI-eGFPnls-T2A-ER-Cre-ER
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
GIT1-Flag/pBK
Plasmid#197313PurposePlasmid expressing an antigen targeting GIT1DepositorInsertGIT1 (Git1 Rat)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOttc1494 - pAAV CaMKII SERCaMP-No Tag
Plasmid#192601PurposeAn AAV packaging vector that expresses SERCaMP under control of the CaMKII promoter.DepositorInsertSERCaMP
UseAAVTagsExpressionMutationPromoterCaMKIIAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRE3GV-somBiPOLES-mKate2
Plasmid#192579PurposeAAV-vector for bidirectional optogenetic manipulation of tTA-positive neuronsDepositorInsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterTRE3GVAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRE3GV-somBiPOLES-mCerulean
Plasmid#192578PurposeAAV-vector for bidirectional optogenetic manipulation of tetTag-positive neuronsDepositorInsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterTRE3GVAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-somBiPOLES-eGFP
Plasmid#192583PurposeAAV-vector for bidirectional optogenetic manipulation of excitatory neuronsDepositorInsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterCaMKIIa(0.4)Available sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLEX-LifeAct-jGCaMP8s-WPRE
Plasmid#186049PurposeAAV-mediated expression of LifeAct-jGCaMP8s under the CAG promoter, Cre-dependent expressionDepositorInsertLifeAct-jGCaMP8s
UseAAVTagsLifeAct-6xHisExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Nup88-V5
Plasmid#175117PurposeLentiviral expression of mouse Nup88-V5DepositorInsertNup88 (Nup88 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Rbmxl1-V5
Plasmid#175124PurposeLentiviral expression of mouse Rbmxl1-V5DepositorInsertRbmxl1 (Rbmxl1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pANY4-pL18-ccdB
Plasmid#164898PurposeTwo valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.DepositorInsertccdB cassette
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP2
Plasmid#166104PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombinationDepositorInsertMsn2-sgRNA#27 (MSN2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP3
Plasmid#166105PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombinationDepositorInsertMsn2-sgRNA#27 (MSN2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP7
Plasmid#166109PurposeThis plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.DepositorInsertWhi5-sg106 (WHI5 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_APL3
Plasmid#166073PurposePlasmid for constituive spCas9 and tet-inducible APL1-targeting sgRNA expression for double stranded break formation in yeastDepositorInsertAPL3 (APL3 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_PPE1
Plasmid#166083PurposePlasmid for constitutive spCas9 and tet-inducible PPE1 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertPPE1 (PPE1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_GAL2
Plasmid#166088PurposePlasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertGAL2 (GAL2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 nickase D10A Cas9 (GB1691)
Plasmid#160588PurposeNickase D10A Cas9 for Nt fusion.DepositorInsertnickase D10A Cas9
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNKR1
Plasmid#67968PurposeHigh copy number plasmid encoding part of the tra Operon PY-traRDepositorInserttra Operon (First Quarter)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNKR2
Plasmid#67969PurposeHigh copy number plasmid encoding part of the tra Operon traC-artADepositorInserttra Operon (second Quarter)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1-rab-3P::his-24::mOrange
Plasmid#124346PurposeNuclear Localized Pan-Neuronal mOrangeDepositorInsertrab-3, mOrange (rab-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only