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Plasmid#158788PurposeRibosome-tagged GCaMP (soma localized) expression in C. elegans ASH neuronsDepositorInsertpsra-6::GCaMP6m::rpl-1a
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS16
Plasmid#158786PurposeRibosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)DepositorInsertPntc-1::GCaMP6m::rpl-1a
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant
Plasmid#101213PurposeBacterial expression plasmid for SpCas9 + K855A variantDepositorInsertSpCas9 variant K855A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationK855APromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiMutB3GALT5catdead-blast
Plasmid#220869Purposelentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequenceDepositorInsertbeta-1,3-galactosyltransferase 5 (B3GALT5 Human)
UseLentiviralTagsmyc, FLAGExpressionMammalianMutationencodes D156A and D243A mutations; also, nucleoti…PromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 mRuby K84ONBK
Plasmid#216226PurposeTDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.DepositorInsertTransactive response DNA binding protein of 43 kDa (TARDBP Human)
UseTagsExpressionMammalianMutationLysine 84 mutated to a TAG stop codonPromoterCMV PromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-Fosprom
Plasmid#188113PurposeExpresses luciferase under control of Fos promoter in transfected cellsDepositorInsertFos promoter (Fos Mouse)
UseLuciferaseTagsExpressionMutationPromoterFos endogenous promoterAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-hCas9-T2A-mStrawberry
Plasmid#187224PurposeExpresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.DepositorInsertCas9
UseCRISPRTagsInserted T2A-mStrawberry at 3' terminal of M…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-SOX2-T2A-miRFP670
Plasmid#187375PurposeExpresses human SOX2 and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertSOX2 (SOX2 Human)
UseTagsT2A-miRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJJGL003
Plasmid#180607PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertionDepositorInsertDpbCasX with R3 loop
UseCRISPRTags10x His and MBPExpressionBacterialMutationPromoterT7Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_162 SpCas9 KES(1107-1109)GG
Plasmid#179526PurposeFor bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tagDepositorInsertSpCas9 KES(1107-1109)GG
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationreplaced KES (residues 1107-1109) with GGPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJCC_164 SpCas9 R1333A/R1335A
Plasmid#179528PurposeFor bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tagDepositorInsertSpCas9 R1333A/R1335A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginines 1333 and 1335 to alaninePromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
sgAAT Nicking
Plasmid#169842PurposeExpress a nicking sgRNA used for correction (via A•T-to-G•C) of the E342K mutationDepositorInsertsgAAT Nicking (SERPINA1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHJ4
Plasmid#158785PurposePan-neuronal nuclear-localized GCaMP expression in C. elegansDepositorInsertPrab-3::nls::GCaMP6m
UseTagsNLSExpressionWormMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
DsRed2-M11-92
Plasmid#134467PurposeM11-92 expressionDepositorInsertM1 Spastin Amino Acids 1-92 (SPAST Human)
UseTagsExpressionMammalianMutationM1 Spastin Amino Acids 1-92PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-M11-92
Plasmid#134465PurposeM11-92 expressionDepositorInsertM1 Spastin Amino Acids 1-92 (SPAST Human)
UseTagsExpressionMammalianMutationM1 Spastin Amino Acids 1-92PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJSC176 - Bacterial expression plasmid for SpCas9 + Q926A variant
Plasmid#101206PurposeBacterial expression plasmid for SpCas9 + Q926A variantDepositorInsertSpCas9 variant Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationQ926APromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA1_no stop
Plasmid#83847PurposeGateway donor vector for IAA1 with no stop codonDepositorInsertIAA1 (IAA1 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
CoV2-M-IRES-E
Plasmid#177938PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.DepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-D614G
Plasmid#177960PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)DepositorInsertSARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
UseTagsExpressionMammalianMutationD614GPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)
Plasmid#197885PurposeCan be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without CreDepositorInsertGFP
UseAAVTagsExpressionMutationN/APromoterCAGAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only