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pAB_PalkB_sfgfp_KanR
Plasmid#166503PurposeExpression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPalkBAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRL25
Plasmid#70253PurposeShuttle vector suitable for transfer to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strainsDepositorTypeEmpty backboneUseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC_sgRNA
Plasmid#68710PurposeContains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-07
Plasmid#124786PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreenDepositorInsertmNeonGreen
UsePcr templateTagsmNeonGreenExpressionMutationPromoterNoneAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
JP1520 PIK3CA E545K HA
Plasmid#14571DepositorInsertPIK3CA E545K (PIK3CA Bovine)
UseRetroviralTagsHA tagExpressionMammalianMutationE545KPromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDM_T7_HisLacZomega
Plasmid#118815PurposeT7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.DepositorInsertlacZ_
UseTagshistagExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-13
Plasmid#119992PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mCherryDepositorInsertmCherry
UsePcr templateTagsmCherryExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B4
Plasmid#31021DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
6x Halo-EGFP- 2x PACT
Plasmid#107265Purpose6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in vitro transcriptionDepositorInsert6x Halo-EGFP- 2x PACT
UseTagsGFPExpressionMammalianMutationPromoterT7Available sinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE30-TEV-pro-conA
Plasmid#159527PurposeExpresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.DepositorArticleInsertCanavalia ensiformis pro-concanavalin A
UseTagsSix-Histidine tag and tobacco etch virus (TEV) pr…ExpressionBacterialMutationPromoterT5Available sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGImC_Empty
Plasmid#92015PurposeEmpty backbone, includes IRES-mCherryDepositorTypeEmpty backboneUseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-23
Plasmid#120002PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with HaloDepositorInsertHalo
UsePcr templateTagsHaloExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTetO-ISce1-TetO
Plasmid#58506PurposeISce1 restriction site is flanked by Tet operator repeats, 96 on each sideDepositorInsertTet repeats flanking ISce1 restriction site
UseTagsExpressionBacterialMutationPromoterno promoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
TwinStrep-SUMO-NLS-BhCas12b_v4
Plasmid#158615PurposeFor protein expression of BhCas12b_v4DepositorInsertNLS-BhCas12b_v4
UseTags6xHIS-TwinStrep-SUMOExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBT-RP2-1
Plasmid#31828DepositorTypeEmpty backboneUseCre/LoxTagsGFP GM2 variant and mRFPExpressionMutationPromoterbeta ActinAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFUS_B10
Plasmid#31027DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MinE-His
Plasmid#133623PurposeExpression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.DepositorInsertMinE-His (minE E. coli)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZ143-pcDNA3-BvCas12b
Plasmid#122445PurposeExpresses BvCas12b in mammalian cellsDepositorInsertCMV-NLS-BvCas12b-NLS-3xHA
UseTags3XHAExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMX1418
Plasmid#127856PurposeExpresses EGFP-IPNN-MCAK in mammalian cellsDepositorInsertIPNN-MCAK
UseTagsEGFPExpressionMammalianMutationmutation is I97N and P98NPromoterCMVAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only
