Addgene

We narrowed to 11,119 results for: 110

Showing: 1421 - 1440 of 11119 results

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Pk_P92-Cd4-bio-His

Plasmid

#126848

Purpose

Expression of recombinant P. knowlesi protein in mammalian cells

Depositor

Gavin Wright

Article

Muller-Sienerth et al Malar J. 2020 Jan 17;19(1):31. doi: 10.1

Insert

P92 (PKH_110660 Plasmodium knowlesi)

Use

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCA13-pHR-SFFV-HaloTag-FBL

Plasmid

#199444

Purpose

Expression of HaloTag-FBL in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

FBL (FBL Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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3xFlag-HaloTag-EZH2 HDR - pDY053

Plasmid

#171108

Purpose

Homology directed repair plasmid for generating HaloTag-EZH2 cells

Depositor

Thomas Cech

Article

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

EZH2 (EZH2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK BirA* 3xFLAG-CSE1L zeo

Plasmid

#192334

Purpose

Lentiviral expression vector for an inducible 3xFLAG-BirA 3xFLAG-CSE1L

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BirA*-3xFLAG-CSE1L (CSE1L Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT BirA*-3xFLAG-CSE1L

Plasmid

#192312

Purpose

Gateway entry vector for an inducible 3xFLAG-BirA* 3xFLAG-CSE1L

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BirA*-3xFLAG-CSE1L (CSE1L Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pKeima-LiveDrop

Plasmid

#199695

Purpose

lipophagy reporter system based on the Keima-fluorophore

Depositor

Tobias Walther

Article

Chung et al Nat Cell Biol. 2023 Aug;25(8):1101-1110.

Insert

GPAT4 (GPAT4 Human)

Use

Tags

Keima

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hcMYC

Plasmid

#206387

Purpose

Expresses human c-MYC in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

c-MYC (MYC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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TLCV2-RB1

Plasmid

#87836

Purpose

LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Insert

sgRB1 (RB1 Human)

Use

CRISPR and Lentiviral; Doxycycline inducible; egf…

Tags

Expression

Mammalian

Mutation

Promoter

Tight TRE promoter and U6

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-c-Myc-L420P

Plasmid

#50776

Purpose

retroviral expression of mouse c-Myc L420P mutant

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

Insert

c-Myc L420P (MYC, Myc Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

L420P (does not bind to Max)

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pET-His6-ERK2-MEK1_R4F_coexpression

Plasmid

#39212

Depositor

Melanie Cobb

Article

Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

Inserts

Mek1 R4F (MAP2K1 Human)
Erk2 (Mapk1 Rat)

Use

Tags

His6 and RBS

Expression

Bacterial

Mutation

MEK1(R4F) = S218E, S222D and deletion of AA32-51

Promoter

T7 and T7 via RBS

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-CIB1-mCerulean-MP

Plasmid

#58366

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Inserts

CIB1[1-147] (CIB1 Mustard Weed)
Camk2a[315-478] (Camk2a Rat)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pEYFP-N3-EPAC1

Plasmid

#113110

Purpose

Human EPAC1 gene was fused in-frame and upstream from the enhanced YFP gene in pEYFP-N3 vector (Clonetech).

Depositor

Xiaodong Cheng

Article

Mei et al J Biol Chem. 2002 Mar 29;277(13):11497-5

Insert

RapGEF3 (RAPGEF3 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

PCMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-CIB1-mRFP1-MP

Plasmid

#58367

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Inserts

CIB1[1-147] (CIB1 Mustard Weed)
Camk2a[315-478] (Camk2a Rat)

Use

Tags

mRFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-TBK1

Plasmid

#23851

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TBK1 (TBK1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-TLK2

Plasmid

#23629

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TLK2 (TLK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PIM2

Plasmid

#23587

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIM2 (PIM2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-SGK2

Plasmid

#23378

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SGK2 (SGK2 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK10

Plasmid

#23437

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK10 (NEK10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pTwist-SARS-CoV-2 Spike-deltaC18 B.1.617v1 (B.1.617.1 v1)

Plasmid

#212428

Purpose

Encodes SARS-CoV-2 spike G142D,E154K,L452R,E484Q,D614G,P681R,Q1071H,H1101D for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike G142D,E154K,L452R,E484Q,D614G,P681R,Q1071H,H1101D (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

G142D,E154K,L452R,E484Q,D614G,P681R,Q1071H,H1101D

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-CIB1-mRuby-Rab35

Plasmid

#194334

Purpose

For use in light-induced Rab35 protein inactivation through the interaction with CRY2

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Inserts

CIB1 (CIB1 Mustard Weed)
Rab35 (RAB35 Human)

Use

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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