-
Plasmid#112659PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 ΔGRM ΔC
UseTagssfGFPExpressionYeastMutationΔ376-493 (deletion of Glo3 motif & C-terminal…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 D
Plasmid#112661PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 S389,398D
UseTagssfGFPExpressionYeastMutationmutations (S389D, S398D) in Glo3 motif, phosphomi…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 Δ2x+ve
Plasmid#112662PurposeExpression S.cerevisiaeDepositorInsertGFP- Glo3 Δ2x+ve
UseTagssfGFPExpressionYeastMutationMutations (R223E, K224E, K225E, K233E, K234E, K23…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1 R54K AxxA
Plasmid#112649PurposeExpression S.cerevisiaeDepositorInsertGcs1 R54K AxxA
UseTagsExpressionYeastMutationR54K & Alanine substitution of C-terminal δL …PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGC684
Plasmid#107022PurposeGFP reporter of lag-2 driven by 1 kb of promoter, which lackes both DBS and Cons motifsDepositorInsertGFP-PH
UseTagsGFPExpressionWormMutationPromoterlag-2Available sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGC682
Plasmid#107020PurposeGFP reporter of lag-2 driven by 1 kb of promoter, which lackes DBS motifDepositorInsertGFP-PH
UseTagsGFPExpressionWormMutationPromoterlag-2Available sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u36_Snifit
Plasmid#119045PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)DepositorInsertMTX-u36_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutation36TAG R44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u51_Snifit
Plasmid#119046PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)DepositorInsertMTX-u51_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutation51TAG R44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MTX-u23_Snifit
Plasmid#119044PurposePlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)DepositorInsertMTX-u23_Snifit
UseTagsHis-tag and Strep-tagExpressionBacterialMutation23TAG R44L H45QPromoterpBADAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-X2DM
Plasmid#115571PurposeGFP-X2 where both of the consensus motif's lysines are mutated to arginineDepositorInsertGreen Fluorescent Protein
UseTagsExpressionBacterial and YeastMutationTwo repeats of the Gcn5 consensus motif are tagge…PromoterADH1Available sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-X2R2
Plasmid#115570PurposeGFP-X2 where the second consensus motif's lysine is mutated to arginineDepositorInsertGreen Fluorescent Protein
UseTagsExpressionBacterial and YeastMutationTwo repeats of the Gcn5 consensus motif are tagge…PromoterADH1Available sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-X2R1
Plasmid#115569PurposeGFP-X2 where the first consensus motif's lysine is mutated to arginineDepositorInsertGreen Fluorescent Protein
UseTagsExpressionBacterial and YeastMutationTwo repeats of the Gcn5 consensus motif are tagge…PromoterADH1Available sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mEGFP
Plasmid#105143PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mEGFP
UseTags81nmt1-mEGFPExpressionBacterial and YeastMutationA206KPromoter81nmt1Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
-
pFloxin-Ofd1-Myc-KDD359-361FSY
Plasmid#24578DepositorInsertOfd1 (Ofd1 Mouse)
UseCre/Lox and Mouse TargetingTagsMycExpressionMutationLysine-Aspartate-Aspartate 359-361 changed to Phe…PromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-hFGD5
Plasmid#50760Purposeexpression of EGFP-tagged human FGD5DepositorInsertFGD5 (FGD5 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA E2F2
Plasmid#24226PurposeMammalian expression of human E2F2 with HA tagDepositorInsertE2F2 (E2F2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMito.iGABASnFR
Plasmid#112175PurposeGABA SensorDepositorInsertiGABASnFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DNAJB1_DNAJ
Plasmid#109918PurposeProtein expression and purification of DNAJB1_DNAJDepositorInsertDNAJB1_DNAJ
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only