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Plasmid#155159PurposeExpresses (Strep-MlaF_L265A)-(6xHis-TEV-MlaB)DepositorInsertMlaF-(6xHis-TEV-MlaB)
UseTagsExpressionMutationMlaF_L265APromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHis6-SUMO-g0s2
Plasmid#179293PurposeE. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage siteDepositorInsertG0S2 (G0S2 Human)
UseTagsHis6 + SUMO + TEV protease cleavage siteExpressionBacterialMutationfull-length G0S2, residues 1-103 from accession n…PromoterT7Available sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pgLAP1
Plasmid#19702DepositorTypeEmpty backboneUseTagsEGFP, S peptide, and TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pgLAP2
Plasmid#19703DepositorTypeEmpty backboneUseTagsFlag, S peptide, and TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
Sc eRF3∆165N (WT)
Plasmid#175418PurposeEncodes His-MBP-TEV-WT eRF3∆165NDepositorInserteRF3 (SUP35 Budding Yeast)
UseTagsHis-MBP-TEVExpressionBacterialMutation∆165NPromoterT7Available sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFB-LIC-Bse
Plasmid#26108PurposeSGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selectionDepositorTypeEmpty backboneUseBaculovirus expressionTagsHis6 - TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceOct. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPe-SH1446
Plasmid#112507PurposeExpresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.DepositorInsertPOT family transporter
UseTagsC terminal TEV GFP octa-His affinity tagExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOCC114
Plasmid#118904Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase siteDepositorInsertNcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with TEV protease, two glycinesExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Syn:NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEcs(ENLYFQ/M)-tTA
Plasmid#135462PurposeNeuronal expression of uFLARE TF componentDepositorInsertNRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEVcs(ENLYFQ/M)-tTA
UseAAVTagsHA, flag, VP16ExpressionMammalianMutationPromoterSynapsinAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18c
Plasmid#135555PurposeExpress mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.DepositorInsertMunc18c
UseTags6xHis tag and TEV siteExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
His-AgdNK
Plasmid#203226PurposeFor protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertdeoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
UseTagsN-terminal TEV cleavable 6xHis tagExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sc NybbR eRF3∆165N (WT)
Plasmid#175419PurposeEncodes His-MBP-TEV-ybbR-WT eRF3∆165NDepositorInserteRF3 (SUP35 Budding Yeast)
UseTagsHis-MBP-TEV and ybbRExpressionBacterialMutation∆165NPromoterT7Available sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDC146
Plasmid#169366PurposeExpresses Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96DepositorInsertSyntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
UseTags6 his tagExpressionBacterialMutationPromoterT7Available sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cas7/pHMGWA
Plasmid#81184PurposeExpresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tagDepositorInsertCas7
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-SGTAP no-pA
Plasmid#82599Purpose3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purificationDepositorInsertSBP-TEV-2x protein G
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cas5c/pHMGWA
Plasmid#81182PurposeExpresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tagDepositorInsertCas5c
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cas8c/pHMGWA
Plasmid#81183PurposeExpresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tagDepositorInsertCas8c
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAP007
Plasmid#206809PurposepET-6xHis-MBP-TEV-BabTIR-APAZ/BabAgo - Heterologous BabSPARTA expressionDepositorInsertBabSPARTA operon (6xHis-MBP-BabTIR-APAZ and BabAgo)
UseTags6xHis-MBPExpressionBacterialMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
12URA-B-ScTop2∆CTD
Plasmid#214924PurposeYeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)DepositorInserttopoisomerase II
UseTags6x His-TEVExpressionYeastMutationDeleted amino acids 1178-1428PromoterGal 1/10Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHYX173
Plasmid#202817PurposePlasmid expressing the TEV protease, codon-optimized for Saccharomyces cerevisiae.DepositorInsertTEV protease
UseTagsExpressionYeastMutationPromoterScPCK1Available sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only