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Showing: 1401 - 1420 of 2594 results

  2. pLV-Azurite

    Plasmid
    #36086
    Purpose
    3rd generation lentiviral plasmid
    Depositor
    Pantelis Tsoulfas
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    36086-plasmid-map-sequence-id-244564

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  6. pJR103

    Plasmid
    #187242
    Purpose
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187242-plasmid-map-sequence-id-374822

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  8. pLVX-H3.3-G34V

    Plasmid
    #209433
    Purpose
    Lentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-G34V with BFP
    Depositor
    Maria Castro
    Insert
    H3.3-G34V
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G34V
    Promoter
    Available since
    Dec. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    209433-plasmid-map-sequence-id-416707

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  9. pcDNA3.1-Zeo-otmyd88

    Plasmid
    #217485
    Purpose
    Expression of Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmon
    Depositor
    Bertrand Collet
    Insert
    BFP-P2A-otPKRFL
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217485-plasmid-map-sequence-id-430348

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  10. CRISPRoff-v2.1

    Plasmid
    #167981
    Purpose
    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Article
    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Insert
    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    Use
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167981-plasmid-map-sequence-id-330241

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  11. pBA950

    Plasmid
    #122239
    Purpose
    CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122239-plasmid-map-sequence-id-292012

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  12. pBA904

    Plasmid
    #122238
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122238-plasmid-map-sequence-id-292011

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  13. pBA900

    Plasmid
    #122237
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs2 at the 3' end of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    122237-plasmid-map-sequence-id-292010

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  14. pJZC116

    Plasmid
    #62344
    Purpose
    sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFP
    Depositor
    Wendell Lim, Stanley Qi
    Article
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 2x MS2 (wt+f6) binding module
    MCP-VP64
    Use
    Lentiviral
    Tags
    VP64
    Expression
    Mammalian
    Mutation
    Targets CXCR4 , sequence: GCAGACGCGAGGAAGGAGGGCGC
    Promoter
    CMV and U6
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62344-plasmid-map-sequence-id-102267

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  15. pPC1000

    Plasmid
    #174829
    Purpose
    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Inserts
    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    EF1a and mouse U6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174829-plasmid-map-sequence-id-343648

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  16. pQ2aB

    Plasmid
    #124887
    Purpose
    Basic Retroviral backbone with multiple 2a sites, linkers, BFP and and IRES_puromycin from pQCXIP
    Depositor
    Nevil Singh
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Blue Flourescent Protein, Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    124887-plasmid-map-sequence-id-243838

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  17. H2B-Electra2

    Plasmid
    #218929
    Purpose
    Electra2 fused to human H2B protein for nuclear localization
    Depositor
    Amro Hamdoun
    Insert
    H2B-Electra2 (H2B Synthetic, Human, Entacmaea quadricolor)
    Use
    Synthetic Biology
    Tags
    Electra2 and H2B
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218929-plasmid-map-sequence-id-433882

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  18. pJH127

    Plasmid
    #162483
    Purpose
    SEC plasmid containing LG1 homology arms and myo-2p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.
    Depositor
    Bob Goldstein
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Use
    CRISPR
    Tags
    myo-2p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    162483-plasmid-map-sequence-id-320838

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  19. pJH130

    Plasmid
    #162484
    Purpose
    SEC plasmid containing LG1 homology arms and myo-3p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.
    Depositor
    Bob Goldstein
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Use
    CRISPR
    Tags
    myo-3p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    162484-plasmid-map-sequence-id-320834

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  20. pJH126

    Plasmid
    #162482
    Purpose
    SEC plasmid containing LG1 homology arms and vha-8p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.
    Depositor
    Bob Goldstein
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Use
    CRISPR
    Tags
    vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    162482-plasmid-map-sequence-id-320829

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Showing: 1401 - 1420 of 2594 results