Addgene

We narrowed to 5,521 results for: #16

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pBBK50 pHomL-RPL18Bp-ARC35-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179034

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ARC35-Link-Neon(gfp), a marker for the actin cytoskeleton

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ARC35-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK48 pHomL-RPL18Bp-SPC42-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179032

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Neon(gfp), a marker for the spindle pole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SPC42-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK47 pHomL-RPL18Bp-SRM1-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179031

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Neon(gfp), a marker for the nucleus

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SRM1-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK46 pHomL-RPL18Bp-RPC19-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179030

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Neon(gfp), a marker for the nucleolus

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-RPC19-Link-Neon(gfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK14 pCAS-Tyr-[gRNA: 4=XII-5] (SplitKanR, AmpR)

Plasmid

#178998

Purpose

Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK13 pCAS-Tyr-[gRNA: 3=XII-2] (SplitKanR, AmpR)

Plasmid

#178997

Purpose

Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR)

Plasmid

#178994

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK09 pCAS-Tyr-[gRNA: BsaI GFP dropout] (BsmBI-flanked URA3, AmpR)

Plasmid

#178993

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = URA3

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)

Plasmid

#178992

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK07 pCAS-Tyr-[gRNA: BsaI GFP dropout] (LEU2,AmpR)

Plasmid

#178991

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = LEU2

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)

Plasmid

#178990

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pKP1693

Plasmid

#167595

Purpose

Express FepA with His6 tag between residues 393 and 394 of the mature amino acid sequence . Please note that mutation numbers are not based on start codon of insert.

Depositor

Kathleen Postle

Article

Gresock et al J Bacteriol. 2017 Apr 25;199(10). pii: J

Insert

FepA

Use

Tags

Expression

Bacterial

Mutation

His6 tag inserted after residue 393 in the mature…

Promoter

Available since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTX1-SMN2-exon7-ESE1

Plasmid

#126042

Purpose

In-vitro-transcription of SMN2-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

SMN2 exon7 ESE1

Use

Tags

Expression

Mutation

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnt2a

Plasmid

#164963

Purpose

Zebrafish kcnt2a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnt2a (kcnt2 Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnma1a

Plasmid

#164951

Purpose

Zebrafish kcnma1a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnma1a (kcnma1a Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR

Plasmid

#157672

Purpose

Expression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

Cohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag

Use

Tags

ddFLN4, ELP, Histag and ybbR tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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p415 Met25 Glo3 ΔGRM-ΔC

Plasmid

#129487

Purpose

Expression in S. cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 ΔGRM ΔC (GLO3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Δ376-493 (deletion of Glo3 motif & C-terminal…

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K ΔGRM-ΔC

Plasmid

#112654

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K ΔGRM ΔC

Use

Tags

Expression

Yeast

Mutation

R59K, Δ376-493 (deletion of Glo3 motif & C-te…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K Δ2x+ve

Plasmid

#112655

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K Δ2x+ve

Use

Tags

Expression

Yeast

Mutation

R59K, mutations (R223E, K224E, K225E, K233E, K234…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K S389,398 D

Plasmid

#112657

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K S389,398D

Use

Tags

Expression

Yeast

Mutation

R59K, mutations (S389D, S398D) in Glo3 motif, pho…

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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