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Plasmid#162249PurposeBlue-light dependent heterodimerization systemDepositorInsertp18-eMagB-iRFP
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLD1 translation factors
Plasmid#117760PurposeBioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coliDepositorInsertTranslation factor cistrons 1-13
UseEscherichia coliTagsExpressionMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
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3XFlag pCMV5-TOPO TAZ (106∂)
Plasmid#24814DepositorInsertTAZ (WWTR1 Human)
UseTags3XFlagExpressionMammalianMutationDeletion of aa 1-106PromoterAvailable sinceMay 14, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLD3 translation factors
Plasmid#117762PurposeBioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coliDepositorInsertTranslation factor cistrons 22-30
UseEscherichia coliTagsExpressionMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLD2 translation factors
Plasmid#117761PurposeBioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coliDepositorInsertTranslation factor cistrons 14-21
UseEscherichia coliTagsExpressionMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
B52_puro_empty_gRNA
Plasmid#197557Purposesites for cloning two gRNA with puromycin cassetteDepositorTypeEmpty backboneUseEmpty grna backbone with puromycin resistance geneTagsExpressionMammalianMutationPromoterU6 for gRNA, CMV for puromycin resistance.Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-subtilase A
Plasmid#35948DepositorInsertSubtilase A (subA E. Coli)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
3XFlag pCMV5-TOPO TAZ (∂WW)
Plasmid#24811DepositorInsertTAZ (WWTR1 Human)
UseTags3XFlagExpressionMammalianMutationDeletion of amino acids 111-158.PromoterAvailable sinceMay 14, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMET7-GAG-GW
Plasmid#194634PurposeGateway destination vector for mammalian expression of Virotrap bait constructsDepositorInsertp55 GAG
UseTagsPQS1ExpressionMammalianMutationPromoterSRalphaAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pBLADE_ONLY_C
Plasmid#168050PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
UseTagsExpressionBacterialMutationPromoterJ23101**Available sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL
Plasmid#47081DepositorInsertAL-09 (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMET7-GAG-HRAS
Plasmid#80604PurposeMammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to itDepositorInsertHIV-1 p55 GAG
UseTagsExpressionMammalianMutationPromoterSRalphaAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTH847-gst-optsc
Plasmid#96908PurposePlasmid for yeast expression of GST encoded by preferred codonsDepositorArticleInsertyeast codon optimised glutathione-S transferase
UseTags8xhistidineExpressionYeastMutationPromoterTDH3Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Codon Optimized Dysferlin
Plasmid#67878PurposeThe human dysferlin cDNA was codon optimized in an attempt to increase Dysferlin production in human cells.DepositorInsertcodon optimized Dysferlin
UseUnspecifiedTagsNoExpressionMutationPromoterNo PromoterAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
TagRFP-T-VAPB(1- 218)- eMagB
Plasmid#162255PurposeBlue-light dependent heterodimerization systemDepositorInsertTagRFP-T-VAPB(1- 218)- eMagB
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOIMars1CyN
Plasmid#75221PurposeMammalian cell surface expression of fluorogen activating protein Mars1CyDepositorInsertss-HA-Mars1Cy-CD80(237-306)
UseTagsHA epitopeExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pP2AeGFP-Mars1Cy
Plasmid#75223PurposeMammalian intracellular expression of fluorogen activating protein Mars1Cy and eGFPDepositorInsertEGFP-p2a-Mars1Cy
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET11a-DAAT-V33G/T242G
Plasmid#159278PurposeAminotransferase variant with near-native catalytic efficiency toward D-tryptophanDepositorInsertEngineered d-alanine aminotransferase
UseTags6x His tagExpressionBacterialMutationValine 33 and threonine 242 have been mutated to …PromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only