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Plasmid#166109PurposeThis plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.DepositorInsertWhi5-sg106 (WHI5 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_APL3
Plasmid#166073PurposePlasmid for constituive spCas9 and tet-inducible APL1-targeting sgRNA expression for double stranded break formation in yeastDepositorInsertAPL3 (APL3 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_PPE1
Plasmid#166083PurposePlasmid for constituive spCas9 and tet-inducible PPE1 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertPPE1 (PPE1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_GAL2
Plasmid#166088PurposePlasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertGAL2 (GAL2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 nickase D10A Cas9 (GB1691)
Plasmid#160588PurposeNickase D10A Cas9 for Nt fusion.DepositorInsertnickase D10A Cas9
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNKR1
Plasmid#67968PurposeHigh copy number plasmid encoding part of the tra Operon PY-traRDepositorInserttra Operon (First Quarter)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNKR2
Plasmid#67969PurposeHigh copy number plasmid encoding part of the tra Operon traC-artADepositorInserttra Operon (second Quarter)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1-rab-3P::his-24::mOrange
Plasmid#124346PurposeNuclear Localized Pan-Neuronal mOrangeDepositorInsertrab-3, mOrange (rab-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS1-lim-4P::his-24::Cerulean
Plasmid#124336PurposeMulticolor Worms for Neuronal IDDepositorInsertlim-4, Cerulean (lim-4 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKPY737
Plasmid#72673PurposeEncodes Thr412Gly-CePheRS Alpha Subunit (Gateway Entry Clone)DepositorInsertThr412Gly-CePheRS Alpha Subunit::C. elegans CEOP5428 Intercistronic Region::HA-GFP [From pPD95.75 (+118 to +1000)] in pDONR221
UseGateway entry cloneTagsExpressionMutationThr412Gly in CePheRS Alpha SubunitPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
psicheck_200_300_CA_repeat
Plasmid#65880PurposeLuciferase assay vector, assessing regulation of 200-300 nucleotide CA repeatsDepositorInsertCA repeats 200-300 nucleotides in length
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
psicheck_300_400_CA_repeat
Plasmid#65879PurposeLuciferase assay vector, assessing regulation of 300-400 nucleotide CA repeatsDepositorInsertCA repeats 300-400 nucleotides in length
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
psicheck_50_100_CA_repeat
Plasmid#65882PurposeLuciferase assay vector, assessing regulation of 50-100 nucleotide CA repeatsDepositorInsertCA repeats 50-100 nucleotides in length
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
3EOZ
Plasmid#51349PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-PGAM2
UseTags6xHisExpressionBacterialMutationTruncation: See CommentsPromoterT7Available sinceFeb. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
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M71 pGL3 Sp5l P1203 mut
Plasmid#17209DepositorInsertSp5l promoter (sp5l Zebrafish)
UseLuciferaseTagsluciferaseExpressionMutation6 of the putative Tcf/Lef sites mutatedPromoterAvailable sinceMay 18, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAM4787
Plasmid#120088PurposeCyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).DepositorInsertPlasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library 1
Pooled Library#168776PurposePooled library expressing barcoded SARS-CoV-2 RNA Binding Domain mutationsDepositorExpressionYeastSpeciesSars-cov-2Available sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library 2
Pooled Library#168777PurposePooled library expressing barcoded SARS-CoV-2 RNA Binding Domain mutationsDepositorExpressionYeastSpeciesSars-cov-2Available sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7TS
Plasmid#17091DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJune 5, 2008AvailabilityAcademic Institutions and Nonprofits only