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Plasmid#101297PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-906 of alpha-CateninPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I affinity mutant (F40-based tension sensor)
Plasmid#119190PurposeThe affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.DepositorInserthuman Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)
Plasmid#101298PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.DepositorInsertalpha-Catenin
UseTagsExpressionMammalianMutationContains amino acids 1-698 of alpha-CateninPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II donor only control (FL-based tension sensor)
Plasmid#118723PurposeThe donor only (YPet(short)) control for the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II-[YPet(short)-FL-mCherry(Y72L)] (internal-1353) (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherry(Y72L)ExpressionMammalianMutationinserted FL-based tension sensor module after aa1…PromoterCMVAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)
Plasmid#166422PurposeExpress pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)DepositorInsertPlasmodium falciparum PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lac1Available sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II donor only control (FL-based no force control)
Plasmid#118722PurposeThe donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry(Y72L)] (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherry(Y72L)ExpressionMammalianMutationtruncation after aa1353; Y72L mutation in mCherry…PromoterCMVAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mSmo
Plasmid#25395DepositorInsertmouse Smoothened cDNA (Smo Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmOrange
Plasmid#37129DepositorInsertLSSmOrange
UseTagsHisExpressionBacterialMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoteraraBAvailable sinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
Desmoglein2-mCherry
Plasmid#36991DepositorInsertDsg2 (DSG2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pH2B-LSSmOrange
Plasmid#37133DepositorInsertLSSmOrange
UseTagshistone 2BExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD-PSmOrange2
Plasmid#34957DepositorInsertPSmOrange2
UseTagsExpressionBacterialMutationR17H, R36H, F64I, Q194L, A224S, G226A (mutations …PromoterAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD-PSmOrange
Plasmid#31886DepositorInsertPSmOrange
UseTagsExpressionBacterialMutationS21T, Q63L, F100Y, L125M, K166R, P192S (mutations…PromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pH2B-PSmOrange2
Plasmid#34960DepositorInsertPSmOrange2
UseTagsH2BExpressionMammalianMutationR17H, R36H, F64I, Q194L, A224S, G226A (mutations …PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPaxillin-PSmOrange
Plasmid#31923DepositorInsertPSmOrange
UseTagsPaxillinExpressionMammalianMutationS21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…PromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVimentin-PSmOrange
Plasmid#31922DepositorInsertPSmOrange
UseTagsVimentinExpressionMammalianMutationS21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…PromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKeratin-PSmOrange
Plasmid#31924DepositorInsertPSmOrange
UseTagsKeratinExpressionMammalianMutationS21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…PromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVimentin-PSmOrange2
Plasmid#34961DepositorInsertPSmOrange2
UseTagsVimentinExpressionMammalianMutationR17H, R36H, F64I, Q194L, A224S, G226A (mutations …PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pH2B-PSmOrange
Plasmid#31920DepositorInsertPSmOrange
UseTagsH2BExpressionMammalianMutationS21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…PromoterAvailable sinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pActinin-LSSmOrange
Plasmid#37139DepositorInsertLSSmOrange
UseTagshuman alpha-actininExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -