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Plasmid#104803PurposeCRISPR/Cas9 2xplex gRNA targeting Medtr4g094545 (Hen1). Also expresses Cas9 from Gmubi promoter.DepositorInsertMedtr4g094545
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC50
Plasmid#104824PurposeCRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.DepositorInsertGlyma.08g081600, Glyma.05g126600
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDM097
Plasmid#216819PurposeAspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.DepositorInsertmStayGold
UseTagsFLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…ExpressionBacterialMutationAspergillus nidulans codon-adjustedPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtGLV1:T35s
Plasmid#164738PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr6g043230.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtGLV9:T35s
Plasmid#164742PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr4g116360.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtGLV10:T35s
Plasmid#164739PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr8g446520.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-col10a1-KI-donor
Plasmid#184874PurposeDonor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repairDepositorInsertsCollagen10a1 5' homology arm
Collagen10a1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
eGFP
UseCRISPR and Cre/LoxTagsExpressionMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pADC10-SETXhel
Plasmid#193056PurposeBaculovirus expression of human Senataxin helicase domain for protein productionDepositorInsertSenataxin (SETX Human)
UseTagsFlagExpressionInsect and MammalianMutationcodon optimized for insect cell expressionPromoterhybrid p10 (insect) and CMV (human) expressionAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
803 pGEX2T PTEN
Plasmid#10784DepositorInsertPTEN (PTEN Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
864 pSG5L HA NLS PTEN
Plasmid#10933DepositorInsertPTEN (PTEN Human)
UseTagsHA and NLSExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
863 pSG5L HA NES PTEN
Plasmid#10932DepositorInsertPTEN (PTEN Human)
UseTagsHA and NESExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
1006 pSG5L Myr HA PTEN
Plasmid#10776DepositorInsertPTEN (PTEN Human)
UseTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
883 pET15b PTEN
Plasmid#10936DepositorInsertPTEN (PTEN Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
994 pSG5L HA PTEN T366A
Plasmid#10788DepositorInsertPTEN (PTEN Human)
UseTagsHAExpressionMammalianMutationT366APromoterAvailable sinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
892 pFastBacHTb PTEN
Plasmid#10938DepositorInsertPTEN (PTEN Human)
UseTagsHisExpressionInsectMutationPromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
918 pSG5L HA PTEN R130G
Plasmid#10781DepositorInsertPTEN (PTEN Human)
UseTagsHAExpressionMammalianMutationR130GPromoterAvailable sinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_4
Plasmid#192688PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_1
Plasmid#192685PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_ZsG gRNA_2
Plasmid#192686PurposeLentiviral expression of Cas9 and ZsGreen1 targeting sgRNADepositorInsertCas9, ZsGreen targeting sgRNA #2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a, U6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only