...genes associated with dementia, such as APP and PSEN1, in most cases the intended HDR events were corrupted...probably due to concomitant NHEJ repair. At the PSEN1 locus, over 90% of the HDR edited alleles had unwanted...

...length polymorphism analysis, allele-specific PCR, T7EN1 cleavage assay, sequencing, RT-qPCR, or immunoblotting...

...gria3a TALEN1258 & TALEN1260 TCGTCCAATAGCTTCTcagtcacgcacgcctgtGAGTTTCTGCTCTTTA gria3a TALEN1258 & TALEN1259...TCGTCCAATAGCTTCTcagtcacgcacgcctgTGAGTTTCTGCTCTTTA gria3a TALEN1295 & TALEN1260 TCGTCCAATAGCTTCTCagtcacgcacgcctgtGAGTTTCTGCTCTTTA...TGACAGCGTTTACTCCACtagaagaagctgtgcgGGAGGATTTCTTTTTATA hey2 TALEN1297 & TALEN1257 TCTTCCGTTTCCACATCCaccacatcccaacagagcAGCGGGAGCAGCAGTAAA...

...bicistronic vector rather than a fusion protein vector) ZsGreen1-N1 - Mammalian Expression SGFP2 495 512 30 Monomer...