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Plasmid#117273PurposeExpresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23ADepositorInsertsUseTagsmScarlet-iExpressionMammalianMutationHuman Sec23A; Sequencing showed a mutation in Sec…PromoterCMV and IRESAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only
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pCDH_TNF-SBP-EGFP
Plasmid#65282PurposeTNF reporter only (no hook) (RUSH system)DepositorInsertTNF (TNF Human)
UseLentiviralTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMutationPromoterCMVAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH_SBP-mCherry-Ecadherin
Plasmid#65291PurposeEcadherin reporter only (no hook) (RUSH system)DepositorInsertEcadherin
UseLentiviralTagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH_TNF-SBP-mCherry
Plasmid#65283PurposeTNF reporter only (no hook) (RUSH system)DepositorInsertTNF (TNF Human)
UseLentiviralTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMutationPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pINTO-N-SA
Plasmid#61723PurposeInducible expression of proteins fused to an N-terminal monomeric streptavidin tag (mSA)DepositorTypeEmpty backboneUseTagsmSAExpressionMammalianMutationPromoterT-REx (CMV + 2xTetO2)Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
TNF-SBP-EGFP_puromycin
Plasmid#65284PurposeTNF reporter only (no hook) (RUSH system)DepositorInsertTNF (TNF Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
TNF-SBP-mCherry_puromycin
Plasmid#65285PurposeTNF reporter only (no hook) (RUSH system)DepositorInsertTNF (TNF Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
ManII-SBP-EGFP_puromycin
Plasmid#65260PurposeManII reporter only (no hook) (RUSH system)DepositorInserttruncated Mannosidae II
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH_ss-SBP-EGFP
Plasmid#172358PurposeReporter to monitoring a streptavidin-binding peptide traffickingDepositorInsertER importing signal sequence
UseLentiviralTagsSBP-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
ManII-SBP-mCherry_puromycin
Plasmid#65261PurposeManII reporter only (no hook) (RUSH system)DepositorInserttruncated Mannosidae II
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ST-SBP-EGFP
Plasmid#65264Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-KDEL and truncated sialyltransferase (ST6GAL1 Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag
Plasmid#216696PurposeSynchronize trafficking of TNFalpha from the ER (RUCH system)DepositorInsertsFIREmate-KDEL
Tumor Necrosis Factor alpha (TNFa)
UseTagsFIREtag, Streptavidin Binding Protein (SBP), and …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ST-SBP-mCherry
Plasmid#65265Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-KDEL and truncated sialyltransferase (ST6GAL1 Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
STR-KDEL_PGRN-SBP-mCherry
Plasmid#180439PurposeCo-expresses (via IRES) streptavidin-KDEL and Progranulin-SBP-mCherryDepositorInsertProgranulin (GRN Human)
UseTagsSBP-mCherryExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ST-SBP-tagBFP
Plasmid#65266Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-KDEL and truncated sialyltransferase (ST6GAL1 Human)
UseTagsStreptavidin Binding Protein (SBP) and tagBFPExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
ST-SBP-EGFP_puromycin
Plasmid#65270PurposeST reporter only ( no hook) (RUSH system)DepositorInserttruncated sialyltransferase (ST6GAL1 Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
ST-SBP-mCherry_puromycin
Plasmid#65271PurposeST reporter only ( no hook) (RUSH system)DepositorInserttruncated sialyltransferase (ST6GAL1 Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/SH/GW-Mst1
Plasmid#84299PurposeExpresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.DepositorInsertMst1 (STK4 Human)
UseTagsHA, SBPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI
Plasmid#216694PurposeSynchronize trafficking of GPI-AP from the ER (RUCH system)DepositorInsertsFIREmate-KDEL
SBP-mApple-FIREtag-GPI
UseTagsFIREtag, IL2 signal sequence, Streptavidin Bindin…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_SBP-mCherry-Golgin84
Plasmid#65304Purposesynchronize trafficking of Golgin-84 from the ER (RUSH system)DepositorInsertGolgin-84 (GOLGA5 Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only