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Plasmid#157244PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertEMB (EMB Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-EMB-Fc(DAPA)-AviTag-6xHis
Plasmid#156680PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertEMB (EMB Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 Assembly Register 3/3 Part - 5'UTR:Luc:Tnos (GB1500)
Plasmid#160581PurposePart 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of Luc and NOS terminatorDepositorInsertLuc
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG membrane-bound lipin-1 (delta200)
Plasmid#120278PurposeExpression of ER/Golgi-tethered lipin-1 in mammalian cellsDepositorInsertLipin-1 (Lpin1 Mouse)
UseTagsFLAG and in-frame fusion with the delta200 Golgi-…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)
Plasmid#163043PurposeQuasAr2-mOrange2 voltage sensor (plasma membrane)DepositorInsertQuasAr2
UseLentiviralTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS010E: Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)
Plasmid#163063PurposeEntry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)DepositorInsertCheRiff
UseGateway entry vectorTagseGFPExpressionMutationPromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator
Plasmid#158585PurposeExpresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiaeDepositorInsertmembrane localization signal-tagBFP-wrmScarlet11
UseTagstagBFPExpressionYeastMutationGGGS linkerPromoterTEFAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)
Plasmid#163062PurposeEntry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)DepositorInsertQuasAr2
UseGateway entry vectorTagsmOrange2ExpressionMutationPromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
PUF Assembly Kit
Plasmid Kit#1000000051PurposeUsed to assemble custom-made RNA-binding proteins for recognition of 8-base long targets within RNA. The kit is based on the Pumilio and FBF (PUF) homology domain of the full-length human Pumilio 1.DepositorAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
FusX TALEN assembly system
Plasmid Kit#1000000063PurposeGolden Gate assembly of 15-16 RVD TALEN inserts in a single cloning step; designed to be used with high throughput roboticsDepositorAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Diatom uLoop Assembly Kit
Plasmid Kit#1000000238PurposeThe collection contains a selection of diatom promoters and terminators from the organism Phaeodactylum tricornutum on vectors compatible with the uLoop cloning system.DepositorAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Start-Stop Assembly Toolkit
Plasmid Kit#1000000152PurposeStart-Stop Assembly is a multi-part, modular DNA assembly method which is functionally scarless, suitable for combinatorial assembly, and ideal for metabolic engineering in diverse organisms.DepositorAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
STAR TALE Assembly Kit
Plasmid Kit#1000000117PurposeThese plasmids use a TALE assembly reaction (STAR) method to construct multiple TALEs in a fast and simple manner.DepositorAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mobius Assembly Vector Toolkit
Plasmid Kit#1000000134PurposeMobius Assembly: Uses a two-level approach for transcriptional unit (TU) and multi-TU augmentation. Each level is comprised of four Acceptor Vectors. Compatible with Golden Gate partsDepositorAvailable sinceMarch 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
MetClo DNA Assembly Kit
Plasmid Kit#1000000153PurposeMetClo DNA Assembly Kit allows modular, hierarchical assembly of DNA fragments using IIS restriction enzyme. Includes 3 E.coli strains for BsaI, BpiI and LguI-based MetClo assembly.DepositorAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Shen Lab DdCBE assembly kit
Plasmid Kit#1000000212PurposeThe DddA-derived cytosine base editor (DdCBE) assembly kit is a collection of 192 modules harboring repeat variable di-residue (RVD) arrays for any desired target.DepositorAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
LIC TAL Effector Assembly Kit
Plasmid Kit#1000000023PurposeLIC (ligation-independent cloning) TALE repeat unit library of 2-mer fragments for assembly in an expression ready TALEN construct in 2 hierarchical assembly steps. Semiâautomated TALE generationDepositorAvailable sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
Multiplex CRISPR/Cas9 Assembly System Kit
Plasmid Kit#1000000055PurposeGolden Gate cloning assembly for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase; see kit #1000000062 for FokI Accessory PackDepositorAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Joung Lab REAL Assembly TALEN Kit
Plasmid Kit#1000000017PurposeUsed to assemble any combination of TALE repeats into arrays of any desired length using the REAL, REAL-Fast or FLASH assembly methods.DepositorAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
Wolfe-Lawson ZFN modular assembly kit
Plasmid Kit#1000000018PurposeContains reagents for engineering zinc finger arrays by the modular assembly method.DepositorAvailable sinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only