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Plasmid#106472PurposeVector for creating C-terminal GFP fusion proteins in yeast.DepositorTypeEmpty backboneUseTagsGFPExpressionBacterial and YeastMutationPromoterNoneAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only
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pDB4697
Plasmid#171125PurposeA plasmid containing the ura4 genomic region,which flanked by a NotI site at the both ends. It served as the control plasmid or backbone for the ura4 integrated plasmid construction.DepositorInsertura4
UseOtherTagsExpressionMutationnon applicablePromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-3HA-His3MX6
Plasmid#41600DepositorInsertHis3
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutationPromoterAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB6574
Plasmid#106132PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPL5618_pUG_MtxR
Plasmid#60929PurposePCR template vector for generating methotrexate resistance cassette.DepositorInsertDihydrofolate reductase (DHFR Human)
UseLoxp flanked deletion cassette templateTagsExpressionMutationL22F F31SPromoterTEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB4848
Plasmid#106138PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH62-EBD
Plasmid#49455Purposeyeast expression of Cre recombinase fused to the estrogen binding domain (EBD), which confers estradiol-dependent control to Cre recombinaseDepositorInsertCre-EBD fusion
UseCre/LoxTagsExpressionYeastMutationPromoterGal1 promoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJH136
Plasmid#47554PurposepJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.DepositorInsertURA3
UsePcr templateTagsExpressionMutationPromoterNative URA3 promoter, -243 to -1.Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYZ959
Plasmid#192603PurposeTEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3DepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsSV40ExpressionYeastMutationPromoterScTEF1Available sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYZ960
Plasmid#187972PurposeTEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with 2µ-URA3DepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsSV40ExpressionYeastMutationPromoterTEF1Available sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB4778
Plasmid#106125PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1-3HA
Plasmid#41609DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTags3xHA, TRP1 reverse orientation, and yeast ADH1 te…ExpressionMutationPromoterAvailable sinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB4666
Plasmid#106144PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB6638
Plasmid#106165PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistanceDepositorInsertunknown
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV0751
Plasmid#133485PurposeExpression in fission yeast from promoter pnmt1 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(nmt1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMC-XI2
Plasmid#176156Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-backbone
Plasmid#172517PurposeYeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertionDepositorInsertgRNA cassette
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB6576
Plasmid#106134PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB6578
Plasmid#106130PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV0752
Plasmid#133486PurposeExpression in fission yeast from promoter pnmt41 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(nmt41)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only