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Showing: 1341 - 1360 of 8122 results
  1. lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

    Plasmid
    #159086
    Purpose
    Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.
    Depositor
    Insert
    empty crRNA-acRNA backbone, dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    EGFP and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    hSYN, U6
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid
    #177360
    Purpose
    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter
    Depositor
    Inserts
    tdTomato
    Chimeric guide for SaCas9
    Use
    AAV
    Tags
    P2A and a tetracycline-controlled transactivator …
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsine promoter and U6 promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid
    #177365
    Purpose
    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression
    Depositor
    Inserts
    tdTomato
    Chimeric guide for SaCas9
    Use
    AAV
    Tags
    P2A and a tetracycline-controlled transactivator …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. Tol2-U6.3-sgRNA-non-targeting-control -GFP

    Plasmid
    #221844
    Purpose
    Tol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter
    Depositor
    Inserts
    EGFP
    non-targeting control sgRNA- GCACTGCTACGATCTACACC
    Use
    CRISPR; Tol2 transposon optimised for chick expre…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    GACG and U6.3 chick
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. lenti-EGFR-L858R-T790M-dual-nick sgRNA

    Plasmid
    #214101
    Purpose
    Lentiviral vector expressing nicking sgRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two sgRNAs using independent U6 promoters.
    Depositor
    Insert
    EGFR L858R nicking sgRNA/EGFR T790M nicking sgRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. sgRNA targeting human SLC12A5 gene stop codon

    Plasmid
    #158577
    Purpose
    For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.
    Depositor
    Insert
    sgRNA targeting human SLC12A5 gene stop codon
    Use
    CRISPR
    Tags
    Cas9 and GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pX330-Flag-evoSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126758
    Purpose
    Expression plasmid for human codon-optimized increased fidelity evoSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Insert
    evoSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126759
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Insert
    HeFSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1341 - 1360 of 8122 results