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Plasmid#113494PurposeExpression of human HOIP with C-terminal strep-HA tagDepositorInsertHOIP (RNF31 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX
Plasmid#75177PurposeNuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.DepositorInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMP71-super-IL-2
Plasmid#174599PurposeExpresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia LabDepositorInsertextracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 mCherry L3-L2
Plasmid#62150PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and mCherry module. Compatible with MultiSite Gateway cloningDepositorInsertmCherry
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterSV40Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEM-PH-citrine
Plasmid#131406PurposePurpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrineDepositorInsertPH
UseIn vitro transcriptionTagscitrine yellow fluorescent proteinExpressionMutationPromoterT7Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMP71-mtGFP-llo-lama4-alg8
Plasmid#174605Purposeexpresses palmytoylated GFP with a C terminal fusion of the LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 and Lama4DepositorInsertpalmytoylGFP w/C-term fusion LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 Lama4
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bin1 delta SH3
Plasmid#168283PurposeExpress SH3-deleted Bin1 mutant (aa 1-404) in pEGFP-N1 vector.DepositorInsertBin1 (Bin1 Mouse)
UseTagsEGFPExpressionMammalianMutationaa 1-404PromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM3D(Gq)::RAM-d2tTA
Plasmid#189627PurposeExpresses hM3D(Gq) under the hSyn promoter, and d2tTA under the pRAM (synthetic Fos) promoter. Plasmid 1 for recording Fos expression.DepositorInsertshM3D(Gq)
d2tTA
UseAAVTagsHAExpressionMammalianMutationPromoterhSyn and pRAMAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3
Plasmid#62131PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseCRISPR; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 Luc2 (FF-Luciferase) L3-L2
Plasmid#62171PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and firefly luciferase module. Compatible with MultiSite Gateway cloningDepositorInsertLuciferase
UseLuciferase; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterSV40Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSC43
Plasmid#104817PurposeCRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from Gmubi promoter from rolD promoterDepositorInsertGlyma.04g057400
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry-CD8-vhhGFP
Plasmid#163930PurposeExpression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PL452-EN1-LA-p2a-mNeonGreen-hGHpA-PGK-neoR-EN1-RA
Plasmid#191684PurposeFor knocking p2a-mNeonGreen into EN1 locusDepositorInsertEN1 homology arms and p2a-mNeonGreen
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PL452-CLSTN2-LA-p2a-EGFP-hGHpA-PGK-neoR-CLSTN2-RA
Plasmid#191688PurposeFor knocking p2a-EFGP into CLSTN2 locusDepositorInsertCLSTN2 homology arms and p2a-EGFP
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR U6 stuffer sgRNA scaffold L1-L4
Plasmid#62128PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloningDepositorTypeEmpty backboneUseCRISPR; Mule gateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ARAF_S214C
Plasmid#51077Purposemammalian expression of ARAF S214CDepositorInsertARAF (ARAF Human)
UseRetroviralTagsExpressionMammalianMutationS214CPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 CreERT2 L3-L2
Plasmid#62174PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and CreERT2 module. Compatible with MultiSite Gateway cloningDepositorInsertCreERT2
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterSV40Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
ama1CD4d3+4-blac
Plasmid#36146PurposeEXPRESs plasmid for P. falciuparum merozoite surface proteins encoding P. falciparum ama1 with rat CD4d3+4-blacDepositorInsertama1 (PF3D7_1133400 Plasmodium falciparum)
UseTagsbeta-lactamase, pentamerization domain of rat car…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBXD1-Strep-HA
Plasmid#113476PurposeExpression of human UBXD1 with C-terminal strep-HA tagDepositorInsertUBXD1 (UBXN6 Human)
UseTags2xstrep-HAExpressionMammalianMutationC174T silent mutation, wildtype without stop codonPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP2_1-395_V
Plasmid#147808PurposeMammalian Expression of HsDCP2_1-395DepositorInsertHsDCP2_1-395 (DCP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only