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Addgene
Showing: 1341 - 1360 of 4623 results

  1. pHH0103 HYPC WW domain #2

    Plasmid
    #104232
    Purpose
    Bacterial expression of WW domain #2 from HYPC
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    HYPC WW domain #2 (PRPF40B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Availability
    Academic Institutions and Nonprofits only
    104232-plasmid-map-sequence-id-204196

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  2. pHH0103 HYPB WW domain

    Plasmid
    #104267
    Purpose
    Bacterial expression of WW domain from HYPB
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    HYPB WW domain (SETD2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Availability
    Academic Institutions and Nonprofits only
    104267-plasmid-map-sequence-id-203491

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  4. pEGB3 omega1 Tnos:BASTA:Pnos - P35S:hCas9:Tnos (GB3469)

    Plasmid
    #160647
    Purpose
    module containing the human codon optimized Cas9 and the BASTA selection marker
    Depositor
    Diego Orzaez
    Insert
    BASTA / Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, P35S
    Availability
    Academic Institutions and Nonprofits only
    160647-plasmid-map-sequence-id-319125

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  5. pSMH1742

    Plasmid
    #160929
    Purpose
    Expression of soluble Sc octamer (Cse4)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    Cse4
    Use
    Tags
    6His-TEV, N-terminal 6His-TEV, and none
    Expression
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    160929-plasmid-map-sequence-id-319094

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  6. pSMH1741

    Plasmid
    #160930
    Purpose
    Expression of soluble Sc octamer (H3)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    HHT1
    Use
    Tags
    6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…
    Expression
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    160930-plasmid-map-sequence-id-318962

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  7. pmCellFree_KA1_SARS-COV-2 _NSP1

    Plasmid
    #169376
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP1 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP1
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Availability
    Academic Institutions and Nonprofits only
    169376-plasmid-map-sequence-id-335277

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  8. pAAV-hPhyB621(Y276H)-P2A-m1V

    Plasmid
    #197377
    Purpose
    The AAV vector for PhyB621(Y276H) and m1V
    Depositor
    Michiyuki Matsuda
    Insert
    Phytochrome B Y276H(1-621 aa)
    Use
    AAV
    Tags
    P2A-m1V
    Expression
    Mutation
    Human codon optimized PhyB (1-621 aa), Y276H muta…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    197377-plasmid-map-sequence-id-389799

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  9. pET3-ARL1-His6 (CO)

    Plasmid
    #201866
    Purpose
    Used for bacterial expression of human protein ARL1
    Depositor
    Richard Kahn
    Insert
    ARL1 (ARL1 Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    201866-plasmid-map-sequence-id-403817

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  10. pET3-ARF6-His6(CO)

    Plasmid
    #201887
    Purpose
    Used for bacterial expression of human protein ARF6
    Depositor
    Richard Kahn
    Insert
    ARF6 (ARF6 Human)
    Use
    Tags
    6xHis (GSGSGSHHHHHH)
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    201887-plasmid-map-sequence-id-403824

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  11. m.3047-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211339
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3047-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    211339-plasmid-map-sequence-id-415656

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  12. m.3075-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211340
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3075-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    211340-plasmid-map-sequence-id-415644

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  13. m.5147-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211341
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.5147-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    211341-plasmid-map-sequence-id-415657

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  14. pET-29b(+)_notufA-CmEndoCOM_3C-CBD-His

    Plasmid
    #218198
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Cordyceps militaris EndoCOM
    Depositor
    Florence Pojer
    Insert
    EndoCOM
    Use
    Tags
    3C-CBD-His
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 19-315
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    218198-plasmid-map-sequence-id-431794

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  18. pFC334

    Plasmid
    #87846
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    yA specific sgRNA
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    A. nidulans gpdA promoter with Hammerhead ribozym…
    Availability
    Academic Institutions and Nonprofits only
    87846-plasmid-map-sequence-id-174613

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  20. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid
    #107941
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R)
    Use
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R and K548R
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    107941-plasmid-map-sequence-id-418295

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Showing: 1341 - 1360 of 4623 results