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Plasmid#217486PurposeExpression of monomeric Enhanced Green Fluorescent ProteinDepositorInsertmEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_GFP
Plasmid#223880Purpose4G-cloning donor for N-terminal GFP tagDepositorInsertGFP
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_GFP-3C
Plasmid#223882Purpose4G-cloning donor for N-terminal GFP-3C tagDepositorInsertGFP-3C
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_GFP
Plasmid#221469PurposeGateway cloning of GFP overexpressionDepositorInsertEGFP
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFPEasy9R
Plasmid#218291PurposePlasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.DepositorInsertpURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4QE2Crimson
Plasmid#218280PurposeIntroduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoterDepositorInsertpURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-DR
Plasmid#216266PurposeDox-responsive module fused with EGFPDepositorInsertCodon-optimized TetR - Lipid binding motif (KRf - KLK) - Codon-optimized TetR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_pLyn_EGFP
Plasmid#214605PurposeExpresses fluorescent protein EGFP targeted to plasma membraneDepositorArticleInsertlyn-EGFP
UseTagslyn targettingExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HT028_FCMV_CheRiff_EGFP
Plasmid#208605PurposeExpressing CheRiff_EGFP under CMV promoterDepositorInsertCheRiff_EGFP
UseLentiviralTagsEGFPExpressionMutationPromoterCMVAvailable sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_T7_gfp
Plasmid#212136PurposeCargo plasmid for integrating PT7 expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertgreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterPT7Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
PgyrA_gfp
Plasmid#212146PurposePlasmid expressing green fluorescent protein under constitutive promoter PgyrA control.DepositorInsertgreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_GFPsp
Plasmid#212144PurposePreparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_GFPsp_CamRcargo
Plasmid#212143PurposePreparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_NEMF_CDKL1
Plasmid#205867PurposeExpress mEGFP-tagged fusion protein, NEMF_CDKL1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_CTNNB1_ULK4
Plasmid#205799PurposeExpress mEGFP-tagged fusion protein, CTNNB1_ULK4 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCM2_I432D_eGFP
Plasmid#208879PurposeExpress eGFP-CCM2 with the I432D mutation in mammalian cells.DepositorArticleInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationmutation I432DPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCM2_W421AD422A_eGFP
Plasmid#208880PurposeExpress eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.DepositorArticleInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationmutations W421A and D422APromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_2T_eGFP
Plasmid#208883PurposeExpress eGFP-TLNRD1 with the L191T and A225T mutations in mammalian cells.DepositorArticleInsertTLNRD1 (TLNRD1 Human)
UseTagseGFPExpressionMammalianMutationmutations L191T and A225TPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_2E_eGFP
Plasmid#208884PurposeExpress eGFP-TLNRD1 with the K192E and R233E mutations in mammalian cells.DepositorArticleInsertTLNRD1 (TLNRD1 Human)
UseTagseGFPExpressionMammalianMutationmutations K192E and R233EPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_5H_eGFP
Plasmid#208861PurposeExpress eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.DepositorArticleInsertTLNRD1 5 helix bundle (TLNRD1 Human)
UseTagsExpressionMammalianMutationDeleted amino acid 142-273PromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only