Addgene

We narrowed to 2,722 results for: Sell

Showing: 671 - 680 of 2722 results

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pCMV-Tag2B EDD C2768A

Plasmid

#37189

Depositor

Darren Saunders, Charles Watts

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD C2768A (UBR5 Human)

Use

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Changed Cysteine 2768 to Alanine

Promoter

CMV

Available since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

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L2_lacZgRNA-Cas9-CsA

Plasmid

#136138

Purpose

Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pJR106

Plasmid

#187244

Purpose

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

Depositor

Jonathan Weissman, Marco Jost

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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EGFR D837A

Plasmid

#11014

Purpose

Retroviral construct for expressing human EGFR with D837A mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D837A (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

D837A

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
EGFR G719S

Plasmid

#11013

Purpose

Retroviral construct for expressing human EGFR with G719S mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR G719S (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

G719S

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pUDC175

Plasmid

#103019

Purpose

S. cerevisae centromic plasmid harboring Fncpf1 under control of TEF1 promoter

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

Fncpf1

Use

Tags

3HA and NLS

Expression

Yeast

Mutation

human codon optimized

Promoter

TEF1

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5 EDD C2768A

Plasmid

#37192

Depositor

Darren Saunders, Charles Watts

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD C2768A (UBR5 Human)

Use

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Changed Cysteine 2768 to Alanine; K503R (please s…

Promoter

SV40

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TUBG2

Plasmid

#171966

Purpose

Expression in cell lines of the TUBG2 gene

Depositor

Maria Alvarado-Kristensson

Article

Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

Insert

TUBG2 (TUBG2 Human)

Use

Tags

Expression

Mammalian

Mutation

Removal of the internal TUBG2-EcoRI site by chang…

Promoter

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-GFP-shRNA-scramble

Plasmid

#182503

Purpose

Expresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter

Depositor

William Wisden

Article

Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

Insert

pan neuronal promoter driving flexed GFP-shRNA scramble from mir 30 cassette

Use

AAV, Cre/Lox, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

hSyn promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pACE-K2P10.1-StrepTagII

Plasmid

#191475

Purpose

Expresses K2P10.1 with C-terminal StrepTagII

Depositor

Arthur Laganowsky

Article

Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

Insert

K2P10.1 (KCNK10, TREK-2 Human)

Use

Tags

Expression

Insect

Mutation

N144Q (uniprot P57789-1)

Promoter

polH

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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