Skip to main content
Addgene
Showing: 1321 - 1340 of 3973 results

  2. 3xmTurquoise2_UtrCH

    Plasmid
    #112963
    Purpose
    In vivo visualization of F-actin (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    UtrCH
    Use
    Tags
    3x mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112963-plasmid-map-sequence-id-220458

    Enlarge

  3. pJYDNgp

    Plasmid
    #162453
    Purpose
    Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-2Glinker-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA nad myc and eUnaG2 fluorescent protein
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162453-plasmid-map-sequence-id-321460

    Enlarge

  5. qTAG-N-Blast-miRFP670nano3

    Plasmid
    #207680
    Purpose
    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miRFP670nano3 cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207680-plasmid-map-sequence-id-413745

    Enlarge

  11. pQE30-TEV-pro-conA

    Plasmid
    #159527
    Purpose
    Expresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.
    Depositor
    Joshua Mylne
    Article
    Nonis et al bioRxiv. 2020.
    Insert
    Canavalia ensiformis pro-concanavalin A
    Use
    Tags
    Six-Histidine tag and tobacco etch virus (TEV) pr…
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    159527-plasmid-map-sequence-id-317076

    Enlarge

  13. pCRISPR-Cas3

    Plasmid
    #209427
    Purpose
    Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes
    Depositor
    Tilmann Weber
    Article
    Whitford et al bioRxiv 2023.05.09.539971
    Insert
    codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PtipA; crRNA under control of PermE*
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209427-plasmid-map-sequence-id-416703

    Enlarge

  15. pUAS-kkv-R896K::NG

    Plasmid
    #138957
    Purpose
    Used to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    kkv-R896K::NG (kkv Fly)
    Use
    Tags
    Neon Green fused to C terminus of Kkv
    Expression
    Insect
    Mutation
    R896K
    Promoter
    UAS
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    138957-plasmid-map-sequence-id-274787

    Enlarge

  16. pJAT56

    Plasmid
    #204300
    Purpose
    FExpress phiC31 integrase under heat shock control. Insert marked with 3XP3-EGFP, which expresses in eyes.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-EGFP
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    3XP3
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204300-plasmid-map-sequence-id-405836

    Enlarge

  19. pJYDN3p

    Plasmid
    #162457
    Purpose
    Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA and myc tags, and eUnaG2 fluorescent…
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162457-plasmid-map-sequence-id-321472

    Enlarge

Showing: 1321 - 1340 of 3973 results