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Plasmid#47903PurposeAn AAV vector that expresses iRFP under the CaM Kinase IIa promoter.DepositorInsertInfrared fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Stop-gcry-mRFP
Plasmid#117772PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsert3x3 Stop sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-N1 human cofilin S3E
Plasmid#50858Purposeexpresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cellsDepositorInsertcofilin 1 S3E (CFL1 Human)
UseTagsmRFPExpressionMammalianMutationS3E psuedo-phosporylated, non-activatablePromoterCMVAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-miRFP670nano3-Blast-H2BC11
Plasmid#207762PurposeDonor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorArticleInsertH2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSB1C3-J23110-B0034-mRFP1_Violet
Plasmid#160447PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Violet
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLin28A-myc-T2A-tagRFP
Plasmid#140267PurposeExpresses LIN28A in mammalian cellsDepositorInsertLIN28A (LIN28A Human)
UseSynthetic BiologyTagsmyc-T2A-tagRFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TDsmURFP-IRES-eGFP
Plasmid#80344PurposeExpresses Tandem Dimer smURFP and eGFP in mammalian cells.DepositorInsertsTandem Dimer smURFP
eGFP
UseTagsExpressionMammalianMutationPromoterCMV and IRESAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO-RFP-IKZF1-sh3
Plasmid#69042PurposeLentiviral expression of IKZF1 shRNADepositorInsertIKZF1 (IKZF1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-tagRFP-NLS-HA-Bxb1
Plasmid#65625PurposeVector for cotranslational expression of tagRFP-Bxb1DepositorInsertBxb1 integrase
UseTagsHA, SV40 NLS, and tagRFPExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHsp83-NLS-HA-2xMCP-2xTagRFP-T
Plasmid#71242PurposeUsed to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.DepositorInsertNLS-2xMCP-2xTagRFP-T
UseP element-mediated germline transformationTagsTagRFP-TExpressionInsectMutationPromoterHsp83Available sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-NFIB-T2A-miRFP670
Plasmid#187222PurposeExpresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertNuclear Factor I B (NFIB Human)
UseTagsInserted T2A-miRFP670 at 3' terminal of MCS.ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
SspB-iRFP-DHPH-TIAM1
Plasmid#176118PurposeHeterodimerization with iLID, Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Tsapphire-StagRFP-SrcAR
Plasmid#173018PurposeLong-Stokes-shift green-red FRET-based Src activity reporter for multiplexed imaging applications.DepositorInsertTsapphire-StagRFP-SrcAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-smURFP-T2A-mCherry
Plasmid#80347PurposeLentiviral transfer vector under the CMV promoter that expresses smURFP and mCherry.DepositorInsertssmURFP
mCherry
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-J23110-B0034-mRFP1_Yellow
Plasmid#160443PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Yellow chromoprotein in E. coliDepositorInsertpromoter, RBS, mRFP1E_Yellow
UseSynthetic BiologyTagsExpressionMutationBioBrick sites removedPromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe RFP1-KRT5 hygro
Plasmid#58493Purposemammalian expression of mRFP1-tagged human KRT5DepositorInsertKRT5 (KRT5 Human)
UseTagsmRFP1ExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-tagRFP-Mad2
Plasmid#114021PurposeLentiviral delivery of tagRFP-MAD2 expressed from the CMV promoterDepositorInserttagRFP-Mad2 (MAD2L1) (MAD2L1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-HP1α-miRFP670-Cry2WT
Plasmid#122442PurposeExpresses disordered protein HP1α fused with fluorescent protein miRFP670 and optogenetic protein Cry2WTDepositorInsertHP1 alpha (CBX5 Human)
UseLentiviralTagsCry2WT and miRFP670ExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fireworks RFP[PTC-] (AVA2515)
Plasmid#85443PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
mRFP-FKBP12-5ptpase domain
Plasmid#67516PurposeRecruitable human type IV 5-phosphatase domain (214-644)DepositorUseTagsmRFPExpressionMutationINPP5E has C641A to destroy the C-terminal CAAX d…PromoterAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only