Addgene

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pBXC3GH

Plasmid

#47070

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pICXNH3

Plasmid

#49020

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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FUGAW

Plasmid

#129473

Purpose

3rd-generation lentiviral vector for hUbC-driven expression of GFP-aequorin

Depositor

Robert Gross

Article

Tung et al Neurophotonics. 2016 Apr;3(2):025001. do

Insert

GFP-aequorin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human ubiquitin C

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-Actin-C-18

Plasmid

#57121

Purpose

Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Use

Tags

mPA-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pRS405-LEU2-TEF-KpolDcr1v2

Plasmid

#85193

Purpose

Integrating plasmid, K. polysporus Dcr1 under TEF promoter

Depositor

David Bartel

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

Dcr1

Use

Integrating, shuttle vector

Tags

Expression

Yeast

Mutation

Promoter

TEF

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV2

Plasmid

#78632

Purpose

GESTALT V2 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT V2 insert

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pYEXC3GH

Plasmid

#49027

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGB-08-09-BECN1

Plasmid

#187991

Purpose

Plasmid for the protein expression of BECN1/Beclin1 in a bacterial and insect expression system. Internal_Reference: SMC1325

Depositor

Sascha Martens

Insert

BECN1 (BECN1 Human)

Use

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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HSA9761

Plasmid

#25486

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

HSA9761 (DIMT1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 23, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pBlue-hFX-HA(NcoI/EcoRI)

Plasmid

#15405

Depositor

Michael Ristow

Insert

human frataxin cDNA (FXN Human)

Use

Tags

AU1 and HA

Expression

Bacterial

Mutation

insert can be released by digestion with REs NcoI…

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pICXC3H

Plasmid

#49022

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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PpurA-cfp

Plasmid

#127936

Purpose

CFP fluorescent reporter for PurA

Depositor

Mary Dunlop

Article

Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

Insert

PpurA-cfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCAndy-CBioTAP

Plasmid

#53570

Purpose

Plasmid vector carrying C-Bio-1xproteinA tag

Depositor

Mitzi Kuroda

Article

Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pYEXNH3

Plasmid

#49024

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pJT103

Plasmid

#25087

Depositor

Christopher Voigt

Article

Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

Insert

PompC-lacZ

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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mEos-A69V-Actin-7

Plasmid

#57334

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Use

Tags

mEos A69V

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pISH-Pknox2

Plasmid

#74354

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pknox2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Pbx/knotted 1 homeobox 2 (Pknox2 )

Use

Tags

Expression

Mutation

Promoter

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Trpm5

Plasmid

#105993

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Trpm5 (Trpm5 Mouse)

Use

To generate riboprobe

Tags

Expression

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pYEXC3H

Plasmid

#49026

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pYEXNHG3

Plasmid

#49025

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge