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Plasmid#108438PurposeStable expression of BAP1-C91A (catalytically inactive mutant) in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.DepositorInsertBAP1 (BAP1 Human)
UseTagsExpressionMammalianMutationC91A: c.271T>G, c.272G>C; others: c.1641C&g…PromoterCMVAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
BAP1-wt (siRNA resistant)
Plasmid#108439PurposeStable expression of BAP1-wt in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.DepositorInsertBAP1 (BAP1 Human)
UseTagsExpressionMammalianMutationc.1641C>T, c.1644T>A, c.1647T>C, c.1650C…PromoterCMVAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-SPRTN-E112A
Plasmid#110215PurposeExpression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsFlagExpressionMammalianMutationE112A, P296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Neo-GFP/V5-Foxa1-(P2A)-Flag-Gata5
Plasmid#105509PurposeMSCV-driven retroviral Foxa1 and Gata5 expression (P2A-linked, V5 and Flag-tagged each)DepositorUseRetroviralTagsFlag and V5ExpressionMutationPromoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)
Plasmid#61364Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLCAR-CD19-28z
Plasmid#135991PurposeModular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterEF1a-shortAvailable sinceJune 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLCAR-CD19-CD3z
Plasmid#135993PurposeModular CD3z alone CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterEF1a-shortAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pSLCAR-CD19-BBz
Plasmid#135992PurposeModular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterEF1a-shortAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ spike_del19
Plasmid#155297PurposeExpression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particlesDepositorInsertSARS-Cov2 spike_deleted (S SARS-Cov2)
UseGeneration of sars-cov-2-pseudotyped lentiviral p…TagsExpressionMutationDeletion in C-term 19 aa of spikePromoterCMVAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro
Plasmid#141395PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInserteGFP
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro
Plasmid#141385PurposeLentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirusDepositorUseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro
Plasmid#141386PurposeLentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirusDepositorUseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro
Plasmid#141391PurposeLentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirusDepositorUseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-nsp5-C145A-2xStrep-IRES-Puro
Plasmid#141371PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp5-C145A (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationC145A, human codon optimizedPromoterEF1alphaAvailable sinceApril 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-Puro
Plasmid#141379PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp13 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro
Plasmid#141380PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp14 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-Puro
Plasmid#141372PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp6 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-Puro
Plasmid#141390PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-orf8 (ORF8 SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro
Plasmid#141373PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits