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Plasmid#183884PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.DepositorInsertACTB sgRNA spacer (ACTB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shSTIM1
Plasmid#89816PurposeKnockdown human STIM1 expressionDepositorInsertSTIM1 shRNA (STIM1 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
KIF1A(1-393)-GCN4-2xmCherry-EF(C)
Plasmid#61665PurposeTandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linkerDepositorInsertKIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
2xmyc-RAB35
Plasmid#164632PurposeExpresses RAB35 in mammalian cells with a 2xmyc tagDepositorInsertRAB35 (RAB35 Human)
UseTags2xmycExpressionMammalianMutationnonePromoterCMVAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB80-hsKIF5B(1-560)Rigor-L-mVenus
Plasmid#193720PurposeExpression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubulesDepositorInserthsKIF5B(1-560)Rigor-L-mVenus (KIF5B Human, Synthetic)
UseTagsmVenusExpressionMammalianMutationhsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …PromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP1
Plasmid#129504PurposeExpresses AvicFP1 constitutively in E. coli (most strains)DepositorInsertAvicFP1
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausGFP
Plasmid#129503PurposeExpresses AausGFP constitutively in E. coli (most strains)DepositorInsertAausGFP
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AvicFP4
Plasmid#129507PurposeExpresses AvicFP4 constitutively in E. coli (most strains)DepositorInsertAvicFP4
UseTagsExpressionBacterialMutationOne of two variants of AvicFP4 tested with essent…Promotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pNCST-AvicFP2
Plasmid#129505PurposeExpresses AvicFP2 constitutively in E. coli (most strains)DepositorInsertAvicFP2
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB80-hsKIF5B(1-560)-Rigor-2xmNeongreen-FRB
Plasmid#193717PurposeRapalog-inducible coupling to human kinesin-1 (KIF5B aa1-560) via FRB to anchor microtubulesDepositorInserthsKIF5B(1-560)-Rigor-2xmNeongreen-FRB (KIF5B Human, Synthetic)
UseTags2xmNeongreen-FRBExpressionMammalianMutationhsKIF5B(aa1-560), RIGOR: G234APromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
JPF0419d
Plasmid#124020PurposeEncodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vectorDepositorInsertPB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
SPRR2A-pFastBac I
Plasmid#182460PurposeExpression His-tagged SPRR2A in insect cellsDepositorInsertsmall proline rich protein 2A (SPRR2A Human)
UseTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
2PH-PLCd-APEX2-P2A-mKate2
Plasmid#71284PurposeMammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporterDepositorInsert2PH-PLCd-APEX2-P2A-mKate2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim1 (Bl/6)
Plasmid#49205Purposeexpresses Flag tagged mouse Tim1DepositorInsertTim1 (BL/6 allele) (Havcr1 Mouse)
UseTagsFLAG and signal sequenceExpressionMammalianMutationTim1 lacks its own signal sequencePromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP2
Plasmid#129500PurposeExpresses AausFP2 constitutively in E. coli (most strains)DepositorInsertAausFP2
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
JPF0535a
Plasmid#124042PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
AKT-APEX2-P2A-mKate2
Plasmid#67654PurposeMammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertAKT-PH-APEX2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-PIF3-mEGFP
Plasmid#139483PurposeExpresses PIF3-mEGFP in mammalian cells.DepositorInsertPIF3 (PIF3 Mustard Weed)
UseLentiviralTagsmEGFPExpressionMammalianMutationPIF3 1-100 aaPromoterHuman EF1a promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP3
Plasmid#129501PurposeExpresses AausFP3 constitutively in E. coli (most strains)DepositorInsertAausFP3
UseTagsExpressionBacterialMutationPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only