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Plasmid#35505PurposeAAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.DepositorHas ServiceAAV9InserthChR2
UseAAVTagsEYFPExpressionMammalianMutationE123APromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
plenti2-EF1a-ChR2-EYFP-WRPE
Plasmid#125255Purpose3rd gen lentiviral expression of ChR2 with mutation fused to EYFP driven by EF1a promoter for optogenetic activationDepositorInserthChR2
UseLentiviralTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA
Plasmid#37087PurposeExpresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for optogenetic inhibitionDepositorInsertNpHR3.0-EYFP
UseAAV and Cre/Lox; Cre-offTagsEYFPExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
HC87 pEYFPN1 anti-Homer1 nanobody
Plasmid#135223PurposeMammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabodyDepositorInsertanti-Homer1 (Mouse) recombinant llama nanobody HC87 (Homer1 L. glama (llama))
UseTags6xHis-HA-EYFPExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-eNpHR 3.0-EYFP
Plasmid#26970Purpose3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by CaMKIIa promoter for optogenetic inhibitionDepositorInserteNpHR 3.0
UseLentiviralTagsEYFPExpressionMammalianMutationTrafficking Signal (TS), ER Export Signal (FCYENE)PromoterCaMKIIaAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHR-MED1trunc-IDR-YFP-NLS
Plasmid#145275PurposeMammalian Expression of Nuclear MED1 truncated IDR-YFP with SV40 NLSDepositorInsertMED1-IDR truncated (MED1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
RV-Cag-Dio-EYFP-P2A-cMaf
Plasmid#98174PurposeRetroviral vector encoding Cre-dependent expression of EYFP-P2A-MafDepositorInsertcMaf (codon optimzed) (Maf Mouse)
UseRetroviralTagsExpressionMutationCodon optimizedPromoterCAGAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-miniTurbo-NES-YFP-STOP-L2
Plasmid#127359Purposegateway entry vector for expressing cytosolic miniTurbo-YFP under a promoter of choiceDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
EYFP-NLS-actin-K68/284R
Plasmid#60616PurposeEncodes YFP and NLS tagged beta-actin with sumoylation resistant mutationsDepositorInsertBeta-actin (ACTB Human)
UseTagsEYFP and NLSExpressionMammalianMutationK68R, 284RPromoterAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS
Plasmid#128833PurposeKnock-in of nuclear EYFP into mouse Elf5 locusDepositorInsertNuclear EYFP into Elf5 locus (Elf5 Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP(1-158)-beta-1 in pcDNAI/Amp
Plasmid#54468PurposeAn amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertYFP (1-158)/beta-1 (GNB1 Human, Aequorea victoria)
UseTagsYFP(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationGBeta-1 was amplified via PCR, which removed an i…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-(STChRger2-TS-EYFP)
Plasmid#129394PurposeSoma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.DepositorInsertsoma targeted ChRger2
UseAAV and Cre/LoxTagsKv2.1-TS-EYFPExpressionMammalianMutationPromoterCAGAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-eArch 3.0-EYFP
Plasmid#35514PurposeLentiviral expression of CaMKIIa-driven Arch 3.0 for optical inhibition.DepositorInserteArch3.0-EYFP
UseLentiviralTagsEYFPExpressionMammalianMutationEnhanced trafficking signal and ER export signal …PromoterCaMKIIaAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-rsChRmine-eYFP-WPRE
Plasmid#183531PurposeOptogeneticsDepositorInsertrsChRmine-eYFP
UseAAVTagsExpressionMutationI146M/G174SPromoterCaMKIIaAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-AP-Smo-YFP-DEST
Plasmid#49099PurposeExpressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell linesDepositorInsertSmo (Smo Mouse)
UseTagsYFP and acceptor peptide (AP)ExpressionMammalianMutationPromoterT7Available sinceNov. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKSB- 3xP3-YFPsv40 GGGG AAGA
Plasmid#174530Purpose3xP3-YFP fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-YFP BsaI cassette
UseTagsExpressionInsectMutationPromoter3xP3Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSyn-GtACR2-ChRmine-tandem-eYFP
Plasmid#192580PurposeAAV-vector for bidirectional optogenetic manipulation of neuronsDepositorInsertsomBiPOLES
UseAAVTagsEYFP- soma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterhuman synapsinAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-Mac 3.0-EYFP
Plasmid#35515PurposeLentiviral expression of CaMKIIa-driven Mac 3.0 for optical inhibition.DepositorInserteMac 3.0-EYFP
UseLentiviralTagsEYFPExpressionMammalianMutationTrafficking signal, ER exportPromoterCaMKIIaAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX601-miniCMV-SaCas9-U6-YFPvsSaCas9 sgRNA
Plasmid#107050PurposeAll-in-one vectors expressing both SaCas9 and YFP sgRNADepositorInsertSaCas9
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-ChETA-EYFP-WPRE-pA
Plasmid#37086PurposeExpresses ChETA-EYFP in cells lacking Cre (Cre-Off), for optogenetic excitationDepositorInsertChETA-YFP
UseAAV and Cre/Lox; Cre-offTagsEYFPExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only