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Plasmid#179014PurposeParent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only
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pBBK29 pHomL-TEF2p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179013PurposeParent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK27 pHomL-RPL18Bp-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179011PurposeParent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS42K_MCP-VP64_D-MS2
Plasmid#177161PurposeExpresses transactivator (MCP-VP64) and scRNA (D-MS2) in yeast cellsDepositorInsertsMCP-VP64
D-MS2
UseTagsSV40 NLSExpressionYeastMutationPromoterADH1 promoter and SNR52 promoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS42K_PCP-VP64_A-PP7
Plasmid#177156PurposeExpresses transactivator (PCP-VP64) and scRNA (A-PP7) in yeast cellsDepositorInsertsPCP-VP64
A-PP7
UseTagsSV40 NLSExpressionYeastMutationPromoterADH1 promoter and SNR52 promoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS42K_MCP-VP64_C-MS2
Plasmid#177160PurposeExpresses transactivator (MCP-VP64) and scRNA (C-MS2) in yeast cellsDepositorInsertsMCP-VP64
C-MS2
UseTagsSV40 NLSExpressionYeastMutationPromoterADH1 promoter and SNR52 promoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046565
Plasmid#149097PurposeYeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
UseTagsCleavable thrombin;StrepIIExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDRF1-GW yosfGFP-T2A-mCherry
Plasmid#118448PurposeProduces equimolar expression of yeast codon-optimized sfGFP and mCherryDepositorInsertyosfGFP-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH825-CEN-minRLuc/slowstaCFLuc
Plasmid#115370PurposeExpresses translation elongation-controlled Renilla luciferase and translation initiation-controlled firefly luciferaseDepositorInsertsGcn4 5'-UTR + Firefly Luciferase
Renilla luciferase (codon minimised)
UseTagsExpressionYeastMutationAll codons have been changed for the least favour…PromoterADH1 and TDH3Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymVenus-T2A-mTurquoise2
Plasmid#118443PurposeProduces equimolar expression of yeast codon-optimized mVenus and mTq2DepositorInsertymVenus-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yomCherry-T2A-mTurquoise2
Plasmid#118447PurposeProduces equimolar expression of yeast codon-optimized mCherry and mTq2DepositorInsertyomCherry-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW yotagRFPT-T2A-mTurquoise2
Plasmid#118449PurposeProduces equimolar expression of yeast codon-optimized tagRFPT and mTq2DepositorInsertyotagRFPT-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-mCFP
Plasmid#105136PurposeYeast gene targetingDepositorInsertkanMX6-P3nmt1-mCFP
UseTags3nmt1-mCFPExpressionBacterial and YeastMutationA206KPromoter3nmt1Available sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-mYFP
Plasmid#105138PurposeYeast gene targetingDepositorInsertkanMX6-P3nmt1-mYFP
UseTags3nmt1-mYFPExpressionBacterial and YeastMutationA206KPromoter3nmt1Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mECitrine
Plasmid#105153PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mECitrine
UseTags81nmt1-mECitrineExpressionBacterial and YeastMutationF64L, Q69M, A206KPromoter81nmt1Available sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 AAAA
Plasmid#87600PurposeEncodes Prp5 AAAA for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationD136A, P137A, L138A, D139APromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 E235A
Plasmid#87601PurposeEncodes Prp5 E235A for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationE235APromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 N399D
Plasmid#87602PurposeEncodes Prp5 N399D for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationN399DPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS413 Prp5 TAG
Plasmid#87603PurposeEncodes Prp5 SAT motif to TAG for expression in yeastDepositorInsertPRP5 (PRP5 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationS446T, T448GPromoterAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 P369E
Plasmid#87277PurposeEncodes Hsh155 P369E for expression in yeastDepositorInsertHSH155 (HSH155 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationP369EPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only