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Plasmid#207678PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mScarlet cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits
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pGL0_19 [panARS ORI]
Plasmid#198932PurposeLevel 0 partDepositorInsertpanARS ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xmNeonGreen_UtrCH
Plasmid#129607PurposeIn vivo visualization of F-actin (can be used for colocalization studies)DepositorArticleInsertUtrophin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mScarlet-Blast
Plasmid#207711PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Blast cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393
Plasmid#216382PurposeBaculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11DepositorInsertTetraspanin-12 (TSPAN12 Human)
UseBaculovirusTags6xHis-3C-splitGFP11ExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-3C-hNorrin(33-133)-1D4_pAcGP67a
Plasmid#216384PurposeBaculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218)DepositorInsertNorrin (NDP Human)
UseBaculovirusTagsGP64 signal sequence-MBP-3C and Rho1D4ExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Pink Flamindo-p2a-bPAC
Plasmid#202716PurposeMammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclaseDepositorArticleInsertsPink Flamindo
bPAC
UseLentiviralTags10xHis and mycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
ITPKA-mNeonGreen
Plasmid#98883PurposeIn vivo visualization of actin cytoskeleton (can be used for colocalization studies)DepositorArticleInsertITPKA
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-MapTau
Plasmid#137808PurposeVisualization of microtubulesDepositorArticleInsertMapTau
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
mNeongreen-PXN
Plasmid#129604PurposeIn vivo visualization of focal adhesions (can be used for colocalization studies)DepositorArticleInsertPaxillin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL0_1 [RK2 ORI]
Plasmid#198918PurposeLevel 0 partDepositorInsertRK2 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
ITPKA_3xmNeonGreen
Plasmid#129606PurposeIn vivo visualization of F-actin (can be used for colocalization studies)DepositorArticleInsertF-tractin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-ultraID
Plasmid#207693PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-ultraID cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-3xHA-Blast
Plasmid#207718PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Blast cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-3xHA-Puro
Plasmid#207729PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-miniTurbo-Puro
Plasmid#207725PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-moxGFP-Blast
Plasmid#207710PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Blast-moxGFP
Plasmid#207677PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-moxGFP cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJAT62
Plasmid#204301PurposeExpress phiC31 integrase under heat shock control. Insert marked with ie1-EGFP, which expresses in abdomen and mouthparts.DepositorInsertie1-EGFP
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-miniTurbo
Plasmid#207692PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miniTurbo cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits