Skip to main content
Addgene
Showing: 1281 - 1300 of 4615 results

  1. KRigor980

    Plasmid
    #129763
    Purpose
    Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    R210A
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129763-plasmid-map-sequence-id-263579

    Enlarge

  5. K560_AviC_coBirA

    Plasmid
    #129765
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129765-plasmid-map-sequence-id-263583

    Enlarge

  10. K560_AviN_coBirA

    Plasmid
    #129766
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129766-plasmid-map-sequence-id-263552

    Enlarge

  11. pTSTEP

    Plasmid
    #72334
    Purpose
    Tissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)
    Depositor
    Avital Rodal
    Article
    Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.
    Insert
    RRS-TagRFPT-p10-[Golic+] RRS-EGFP
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    72334-plasmid-map-sequence-id-132006

    Enlarge

  12. pDsRed-TSTEP_v2

    Plasmid
    #72336
    Purpose
    Tissue-specifically swappable (C-terminal TagRFPT-to-EGFP) genome tagging plasmid with dsRed selection marker for injection mediated gene targeting (Drosophila melanogaster)
    Depositor
    Avital Rodal
    Article
    Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.
    Insert
    RRS-TagRFPT-p10-[3xP3dsRed]-RRS-EGFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    72336-plasmid-map-sequence-id-132003

    Enlarge

  14. pMT-HA-STX1

    Plasmid
    #89310
    Purpose
    Expresses HA tagged syntaxin 1 in Drosophila cells
    Depositor
    Andrew Peden
    Insert
    STX1 (Syx1A Fly)
    Use
    Tags
    HA tag
    Expression
    Insect
    Mutation
    Promoter
    P-MT (Metallothionein)
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    89310-plasmid-map-sequence-id-190170

    Enlarge

  15. CvcE in pGEX4t

    Plasmid
    #112977
    Purpose
    For protein expression and purification of Drosophila CvcE
    Depositor
    Terry Orr-Weaver
    Insert
    cycE (CycE Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    112977-plasmid-map-sequence-id-217475

    Enlarge

  18. K406

    Plasmid
    #129764
    Purpose
    Expresses Drosophila Kinesin-1 truncated after neck-coil at 406
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 406
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129764-plasmid-map-sequence-id-263574

    Enlarge

Showing: 1281 - 1300 of 4615 results