Addgene

We narrowed to 5,521 results for: #16

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pRN3P_T3_BE4_IVT

Plasmid

#171762

Purpose

Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase

Depositor

Timo Otonkoski

Article

Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

Insert

BE4

Use

Vector for in-vitro transcription

Tags

Expression

Mutation

Promoter

T3 promoter

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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3535 pET15b Bid p22

Plasmid

#8784

Depositor

Stanley Korsmeyer

Article

Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

Insert

Bid p22 (Bid Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pGL1.7XX

Plasmid

#49344

Purpose

luciferase reporter with mouse Bmp2 promoter

Depositor

Melissa Rogers

Article

Abrams et al J Biol Chem. 2004 Apr 16;279(16):15916-2

Insert

Bmp2 distal promoter (Bmp2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mutation

nt –1237 to +471

Promoter

Bmp2 distal promoter

Available since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pBBK86 pHomL-RPL18Bp-preCOX4-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179070

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of preCOX4-Link-Scarlet(rfp), a marker for the mitochondria

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-preCOX4-Link-Scarlet(rfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK80 pHomL-RPL18Bp-Scarlet(rfp)-Link-VAM3-SSA1t-HomR (AmpR)

Plasmid

#179064

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VAM3, a marker for the vacuole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-VAM3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK68 pHomL-RPL18Bp-SRM1-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179052

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Scarlet(rfp), a marker for the nucleus

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SRM1-Link-Scarlet(rfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTRF.1-mCMV-udsVenus

Plasmid

#58518

Purpose

Empty lentiviral expression vector for ultradestabilized Venus that indicates the activity of a transcriptional element of interest

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

None

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-f:PGC1(delta CTD)

Plasmid

#1030

Depositor

Bruce Spiegelman

Article

Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

Insert

PGC1a (Ppargc1a Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

delta CTD

Promoter

Available since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Hmga2 m7

Plasmid

#14792

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Hmga2 m7 (Hmga2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Hmga2 m7 mutant (see article and author's ma…

Promoter

Available since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pEBG-TFII-I N90

Plasmid

#22186

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2001 Mar 16. 276(11):8377-8

Insert

TFII-I (GTF2I Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Contains the first 90 aa of TFII-I

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-dsRed

Plasmid

#73460

Purpose

Expresses dsRed for monosynaptic circuit mapping

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

dsRed

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pFBD-ESCRT 0

Plasmid

#21499

Depositor

James Hurley

Article

Ren et al Structure. 2009 Mar 11. 17(3):406-16.

Insert

pFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Human, Mouse)

Use

Tags

GST and His-MBP

Expression

Insect

Mutation

none

Promoter

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
RabV CVS N2c(deltaG)-synaptophysin-GFP

Plasmid

#73492

Purpose

Expresses EGFP for monosynaptic circuit mapping at the synapse

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

synaptophysin-GFP

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSR9.924

Plasmid

#39539

Depositor

Katharina Strub

Article

Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

Insert

SRP9 (SRP9 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pET28 Set9 mutant

Plasmid

#24083

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Use

Tags

FLAG and His

Expression

Bacterial

Mutation

H297A

Promoter

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pSR9.1347

Plasmid

#39540

Depositor

Katharina Strub

Article

Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

Insert

SRP9 (SRP9 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
MS2_adRNA-MCP_ADAR2 DD_NLS

Plasmid

#124704

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+1D

Plasmid

#149389

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Use

Tags

Expression

Bacterial

Mutation

1bp inserted downstream of the IHFprox site

Promoter

pBAD

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pXSE901B

Plasmid

#91713

Purpose

CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance

Depositor

Qi-Jun Chen

Article

Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBBK92 pHomL-TDH3p-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

Plasmid

#179076

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulum

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Scarlet(rfp)-Link-SCS2tmh

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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