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Plasmid#80970PurposeCRISPaint gene taggingDepositorInsertTagGFP2
UseGene taggingTagsExpressionMutationPromoternoneAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro SHP2 WT
Plasmid#8329DepositorInsertSHP2 (PTPN11 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2005AvailabilityAcademic Institutions and Nonprofits only -
OCT4-eGFP-2A-Puro
Plasmid#31939DepositorUseTargeting donorTagseGFPExpressionMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Puro-ARHGAP26
Plasmid#69467PurposeExpresses ARHGAP26DepositorInsertARHGAP26 (ARHGAP26 Human)
UseRetroviralTagsHA TAGExpressionMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_sgRNA_puro-mKate-lox5171
Plasmid#162077Purpose3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistanceDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-MEK-DD
Plasmid#15268DepositorInsertMap2k1 (Mapk1 Mouse)
UseRetroviralTagsExpressionMammalianMutationS218D S222DPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
Ki67-mNeonGreen-IRESpuro2
Plasmid#183737PurposeExpresses Ki67-mNeonGreenDepositorInsertMKI67 (MKI67 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiX2-PURO-shLKB1-Hu
Plasmid#61242PurposeLentivirus expressing shRNA to human LKB1DepositorInsertLKB1 (STK11 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSico PGK puro
Plasmid#11586DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSicoR PGK puro
Plasmid#12084DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-REDD1-HA
Plasmid#133550PurposeExpresses HA tagged REDD1 in Mammalian cellsDepositorInsertRegulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)
UseRetroviralTagsHA tagExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-shFAM134B
Plasmid#109013Purposeshort-hairpin knockdown of targeted geneDepositorInsertshFAM134B (RETREG1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cas12j-8-puro-crRNA
Plasmid#194965PurposeThe plasmid expresses human codon-optimized Cas12j-8, crRNA expression elements and puromycin resistence gene.DepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR
Plasmid#71667PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, S. pyogenes, Human)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti_gRNA -hMLHdn-Puro
Plasmid#191105PurposeBackbone for lentiviral pegRNA library construction with hMLH1dn and Puro markerDepositorInserthMLH1dn
UseCRISPR and LentiviralTagsExpressionMammalianMutationI31 silent mutation (ATA to ATC) to remove early …PromoterEF-1aAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
VSVGwt-SBP-EGFP_puromycin
Plasmid#65302PurposeVSVG reporter only (no hook) (RUSH system)DepositorInsertVSV-G
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-HaloTag-PuroR
Plasmid#80960PurposeCRISPaint gene taggingDepositorInsertHaloTag
UseGene taggingTagsExpressionMutationPromoternoneAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-PCNA-PuroR
Plasmid#215063PurposeExpresses mCerulean-PCNA in mammalian cells from a retroviral vector.DepositorInsertPCNA (PCNA Human)
UseRetroviralTagsmCeruleanExpressionMammalianMutationPromoterLTRAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-CRX-T2A-NeuroD1
Plasmid#194607PurposePiggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.DepositorInsertCRX-T2A-NeuroD1
UsePiggybac transposonTagsExpressionMammalianMutationPromotertetOAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiX2-PURO-shGFP
Plasmid#61230PurposeLentivirus expressing shRNA to GFPDepositorInsertGFP
UseLentiviralTagsExpressionMutationPromoterH1/TOAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only