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Plasmid#63076PurposeRetroviral expression of EGFP-TEV-S-tag FANC-A for protein localization and affinity purification (LAP)DepositorInsertFANC-A, IMAGE clone 5725803 (FANCA Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIC84
Plasmid#63062PurposeRetroviral expression of EGFP-TEV-S-tag hCENP-H for protein localization and affinity purification (LAP)DepositorInserthCENP-H, IMAGE clone 3451767 (CENPH Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIC146
Plasmid#63069PurposeRetroviral expression of EGFP-TEV-S-tag CENP-P for protein localization and affinity purification (LAP)DepositorInsertCENP-P, IMAGE clone 5554572 (CENPP Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJK569
Plasmid#72455PurposeProduces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic geneDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationsynthetic gene with ~51 silent substitutionsPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
P25-pZE-SUMO-PbTTA
Plasmid#204634PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized pbTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-PbTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
P14-pZE-SUMO-ObiH
Plasmid#204629PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized obiH gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-ObiH
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
P15-pZE-SUMO-PiTTA
Plasmid#204630PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized piTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-BuTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
P17-pZE-SUMO-CsTTA
Plasmid#204631PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized csTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-CsTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
P18-pZE-SUMO-BuTTA
Plasmid#204632PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized buTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-BuTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
P24-pZE-SUMO-KaTTA
Plasmid#204633PurposeColE1 ori, KanR, TetR, Tet promoter with a codon optimized kaTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.DepositorInsertHis-SUMO-TEV-KaTTA
UseSynthetic BiologyTagsHis Tag; SUMO Tag; TEV Protease SiteExpressionBacterialMutationPromoterPLtetO-1 promoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4C(G154C)
Plasmid#190783PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, G154C)DepositorInsertGB1-His6-TEV-CHMP4C(121-233, G154C) (CHMP4C Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationG154CPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4C(M165C)
Plasmid#190784PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, M165C)DepositorInsertGB1-His6-TEV-CHMP4C(121-233, M165C) (CHMP4C Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationM165CPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ΔN-CHMP4C(S191C)
Plasmid#180027PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C).DepositorInsertGB1-His6-TEV-CHMP4C(121-233, S191C) (CHMP4C Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationS191CPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human Lipid Droplet and Metabolism Library
Pooled Library#191535PurposeLentiviral CRISPR library targeting 1,196 genes related to lipid droplet biology and cell metabolism.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIC80
Plasmid#63064PurposeMammalian expression of EGFP-TEV-S-tag DSN1 for protein localization and affinity purification (LAP)DepositorInsertDSN1, IMAGE clone 6063417 (DSN1 Human)
UseTagsEYFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMLS382
Plasmid#73731PurposeSapTrap Flagtag-TEV C-terminal connector donor plasmidDepositorInsertFLAGtag-TEV
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMLS383
Plasmid#73736PurposeSapTrap TEV-Flagtag N-terminal connector donor plasmidDepositorInsertTEV-FLAGtag
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIC145
Plasmid#63068PurposeRetroviral expression of EGFP-TEV-S-tag CENP-O for protein localization and affinity purification (LAP)DepositorInsertCENP-O, IMAGE clone 3942844 (CENPO Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
avi-eTMPRSS11D S368A
Plasmid#220882PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertTMPRSS11D (TMPRSS11D Human)
UseBaculovirus expresssionTagsHoneybee melittinsignalExpressionMutationS368APromoterPolyhedrinAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMta2-EosHalo-TAFiP
Plasmid#140959PurposeTargeting construct to make Mta2-Eos-Halo-Tev-Avi-3xFlag fusionDepositorInsertmetastasis-associated gene family, member 2 (Mta2 Mouse, Synthetic)
UseMouse TargetingTags3xFLAG, Avi, Eos, Halo, and TEVExpressionMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only