Addgene

We narrowed to 1,935 results for: eda

Showing: 1261 - 1280 of 1935 results

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pSpCas9-Puro HLAG-2B-sgRNA

Plasmid

#182552

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Article

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2B-sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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FLI1_pet28a

Plasmid

#131641

Purpose

Bacterial expression of ETS gene FLI1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

FLI1 (FLI1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-RNF8-N-terminus(1-140)

Plasmid

#64675

Purpose

expresses only N terminus of RNF8, amino acids 1 to 140

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Ring finger protein 8 (RNF8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

only N terminus amino acids 1-140

Promoter

CMV

Available since

May 20, 2015

Availability

Academic Institutions and Nonprofits only
ELF2_pet28a

Plasmid

#131662

Purpose

Bacterial expression of ETS gene ELF2

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF2 (ELF2 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pClneoMyc MOB1 T74A

Plasmid

#47939

Purpose

expresses Myc tagged human MOB1 containing T74A mutation

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T74A

Promoter

CMV

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
px335-sgRNA-EF(Cas9wt)-Rif1

Plasmid

#122306

Purpose

Expresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Rif1 (Rif1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-WRAP53beta-DC-mutant-G435R

Plasmid

#64684

Purpose

expresses single mutated WRAP53beta at G435R, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutation at G435R

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-DC-mutant-R398W

Plasmid

#64683

Purpose

expresses single mutated WRAP53beta at R398W, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutation at R398W

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pNIC28-BSA4-G-S18

Plasmid

#127825

Purpose

Production of recombinant human ribosomal protein S18

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS18 (RPS18 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF66-2xStrep

Plasmid

#130953

Purpose

Expresses KSHV ORF66 a.a. 1-429 (full length) with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

Insert

ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))

Use

Tags

2xStrep

Expression

Mammalian

Mutation

ORF66 a.a. 1-429 (full-length)

Promoter

CMV

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pClneoMyc MOB1 T12A

Plasmid

#47936

Purpose

expresses Myc tagged human MOB1 containing T12A mutation

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T12A

Promoter

CMV

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-DC-mutant-F164L

Plasmid

#64681

Purpose

expresses single mutated WRAP53beta at F164L, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutation at F164L

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-DC-mutant-H376Y

Plasmid

#64682

Purpose

expresses single mutated WRAP53beta at H376Y, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutation at H376Y

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
px335-EF-Slc2a3-R

Plasmid

#122305

Purpose

Expresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Slc2a3 (Slc2a3 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2c-W290C

Plasmid

#45701

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIc (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

W290C

Promoter

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2b-P253R

Plasmid

#45716

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

P253R

Promoter

Available since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2c-K660N

Plasmid

#45708

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIc (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

K660N

Promoter

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
p3xFLAG-CMV-RNF8-N-terminus(1-111)

Plasmid

#64674

Purpose

Expresses only N terminus of RNF8, amino acids 1 to 111

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Ring finger protein 8 (RNF8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Only N terminus amino acids 1-111

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
p28BIOHTEV-LIC: nsp16_SARS-CoV-2

Plasmid

#167846

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp16

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp16

Use

Tags

6xHis and AviTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBp-FGFR2b-W290C

Plasmid

#45700

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

W290C

Promoter

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only